Detailed information of TA system TA209469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 5150041..5150540
• Replicon: chromosome
• Accession: NZ_CP079225
• Organism: Rhodococcus sp. LW-XY12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H0JMW2
• Locus tag: KXC42_RS23955
• Protein ID: WP_006550854.1
• Coordinates: 5150041..5150295 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H0JMW3
• Locus tag: KXC42_RS23960
• Protein ID: WP_006550855.1
• Coordinates: 5150292..5150540 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXC42_RS23925 (KXC42_23910)	5145475..5146644	-	1170	WP_019290521.1	acyl-CoA dehydrogenase family protein	-
KXC42_RS23930 (KXC42_23915)	5146747..5147373	+	627	WP_006550849.1	TetR family transcriptional regulator	-
KXC42_RS23935 (KXC42_23920)	5147531..5147743	-	213	WP_019290520.1	hypothetical protein	-
KXC42_RS23940 (KXC42_23925)	5147750..5147983	-	234	WP_257606203.1	transposase	-
KXC42_RS23945 (KXC42_23930)	5148306..5149262	-	957	WP_039584493.1	ERCC4 domain-containing protein	-
KXC42_RS23950 (KXC42_23935)	5149419..5149940	+	522	WP_006550853.1	hypothetical protein	-
KXC42_RS23955 (KXC42_23940)	5150041..5150295	-	255	WP_006550854.1	Txe/YoeB family addiction module toxin	Toxin
KXC42_RS23960 (KXC42_23945)	5150292..5150540	-	249	WP_006550855.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KXC42_RS23965 (KXC42_23950)	5150636..5150905	+	270	WP_019290514.1	pirin-like C-terminal cupin domain-containing protein	-
KXC42_RS23970 (KXC42_23955)	5151372..5152166	+	795	WP_006550857.1	LLM class F420-dependent oxidoreductase	-
KXC42_RS23975 (KXC42_23960)	5152227..5153459	+	1233	WP_219129717.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10064.34 Da
• Isoelectric Point: 6.7171

>T209469 WP_006550854.1 NZ_CP079225:c5150295-5150041 [Rhodococcus sp. LW-XY12]
VNITFTDEAWDDYQFWISTDRKQLERVNELVRDCQRDPYSGIGKPERLRHALSGLWSRRIDKEHRLVYEVIDGALVIIAA
RYHY

Nucleotide

• Length: 255 bp

>T209469 NZ_CP104114:445984-446157 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.59 Da
• Isoelectric Point: 5.8315

>AT209469 WP_006550855.1 NZ_CP079225:c5150540-5150292 [Rhodococcus sp. LW-XY12]
MAAITATEARKNFFPLLEQVNKDHTPVEIVSKRGNAVLMSKDDYDAMLETSYLMQSPANARRLLASMQRARGGEYTEREL
IE

Nucleotide

• Length: 249 bp

>AT209469 NZ_CP104114:445660-445956 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: H0JMW2

Antitoxin

• AlphaFold DB: H0JMW3