Detailed information of TA system TA209460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 2226593..2227148
• Replicon: chromosome
• Accession: NZ_CP079202
• Organism: Bifidobacterium longum subsp. suillum strain KCTC 15605

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BLS605_RS09540
• Protein ID: WP_197308618.1
• Coordinates: 2226593..2226916 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2ZDC4
• Locus tag: BLS605_RS09545
• Protein ID: WP_003829112.1
• Coordinates: 2226903..2227148 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLS605_RS09515 (BLS605_09515)	2221707..2222801	+	1095	WP_197308615.1	redox-regulated ATPase YchF	-
BLS605_RS09520 (BLS605_09520)	2222957..2224222	-	1266	WP_197308616.1	Fic family protein	-
BLS605_RS09525 (BLS605_09525)	2224767..2225132	-	366	WP_012577984.1	YccF domain-containing protein	-
BLS605_RS09530 (BLS605_09530)	2225369..2225728	+	360	WP_231691745.1	class I SAM-dependent methyltransferase	-
BLS605_RS09535 (BLS605_09535)	2225876..2226376	+	501	WP_197308617.1	cupin domain-containing protein	-
BLS605_RS09540 (BLS605_09540)	2226593..2226916	-	324	WP_197308618.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLS605_RS09545 (BLS605_09545)	2226903..2227148	-	246	WP_003829112.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLS605_RS09550 (BLS605_09550)	2227347..2228444	+	1098	WP_231691746.1	HipA domain-containing protein	-
BLS605_RS10355	2228544..2228900	+	357	WP_231691747.1	hypothetical protein	-
BLS605_RS09560 (BLS605_09560)	2229084..2230355	-	1272	WP_197308619.1	MFS transporter	-
BLS605_RS09565 (BLS605_09565)	2230512..2231174	-	663	WP_197308644.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11840.62 Da
• Isoelectric Point: 5.0611

>T209460 WP_197308618.1 NZ_CP079202:c2226916-2226593 [Bifidobacterium longum subsp. suillum]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGHQLMRVLGLL

Nucleotide

• Length: 324 bp

>T209460 NZ_CP104112:c3559639-3559304 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9339.43 Da
• Isoelectric Point: 4.5881

>AT209460 WP_003829112.1 NZ_CP079202:c2227148-2226903 [Bifidobacterium longum subsp. suillum]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT209460 NZ_CP104112:c3559887-3559639 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A087B6Q6