Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5342377..5343002 Replicon chromosome
Accession NZ_CP079186
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC14_AI2040MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWZ53_RS26010 Protein ID WP_002882817.1
Coordinates 5342377..5342760 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWZ53_RS26015 Protein ID WP_004150355.1
Coordinates 5342760..5343002 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWZ53_RS25995 (5339743) 5339743..5340645 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWZ53_RS26000 (5340642) 5340642..5341277 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWZ53_RS26005 (5341274) 5341274..5342203 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KWZ53_RS26010 (5342377) 5342377..5342760 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWZ53_RS26015 (5342760) 5342760..5343002 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWZ53_RS26020 (5343207) 5343207..5344124 + 918 WP_004181612.1 alpha/beta hydrolase -
KWZ53_RS26025 (5344138) 5344138..5345079 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWZ53_RS26030 (5345124) 5345124..5345561 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KWZ53_RS26035 (5345558) 5345558..5346418 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWZ53_RS26040 (5346412) 5346412..5347011 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209449 WP_002882817.1 NZ_CP079186:c5342760-5342377 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209449 NZ_CP104112:920530-920928 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209449 WP_004150355.1 NZ_CP079186:c5343002-5342760 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209449 NZ_CP104112:920234-920527 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References