Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4724847..4725657 Replicon chromosome
Accession NZ_CP079186
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC14_AI2040MC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWZ53_RS23130 Protein ID WP_004178461.1
Coordinates 4724847..4725380 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWZ53_RS23135 Protein ID WP_002887278.1
Coordinates 4725391..4725657 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWZ53_RS23125 (4723678) 4723678..4724799 + 1122 WP_004214138.1 cupin domain-containing protein -
KWZ53_RS23130 (4724847) 4724847..4725380 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWZ53_RS23135 (4725391) 4725391..4725657 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWZ53_RS23140 (4725760) 4725760..4727193 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
KWZ53_RS23145 (4727183) 4727183..4727866 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWZ53_RS23150 (4728038) 4728038..4729423 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
KWZ53_RS23155 (4729441) 4729441..4729785 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209447 WP_004178461.1 NZ_CP079186:c4725380-4724847 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209447 NZ_CP104112:426804-426977 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209447 WP_002887278.1 NZ_CP079186:c4725657-4725391 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209447 NZ_CP104112:426480-426776 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References