Detailed information of TA system TA209444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2784785..2786373
• Replicon: chromosome
• Accession: NZ_CP079186
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC14_AI2040MC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KWZ53_RS13715
• Protein ID: WP_064162061.1
• Coordinates: 2784785..2786107 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: KWZ53_RS13720
• Protein ID: WP_002904790.1
• Coordinates: 2786107..2786373 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWZ53_RS13700 (2779841)	2779841..2782342	+	2502	WP_218415635.1	fimbria/pilus outer membrane usher protein	-
KWZ53_RS13705 (2782330)	2782330..2783334	+	1005	WP_064162060.1	fimbrial protein	-
KWZ53_RS13710 (2783476)	2783476..2784768	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KWZ53_RS13715 (2784785)	2784785..2786107	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
KWZ53_RS13720 (2786107)	2786107..2786373	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KWZ53_RS13725 (2786639)	2786639..2786965	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
KWZ53_RS13730 (2786962)	2786962..2787462	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KWZ53_RS13735 (2787440)	2787440..2788819	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
KWZ53_RS13740 (2789165)	2789165..2790319	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KWZ53_RS13745 (2790300)	2790300..2791223	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T209444 WP_064162061.1 NZ_CP079186:c2786107-2784785 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209444 NZ_CP104110:c4808996-4808712 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT209444 WP_002904790.1 NZ_CP079186:c2786373-2786107 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209444 NZ_CP104110:c4809228-4808986 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8