Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53147..53672 Replicon plasmid pAI2040P_P2
Accession NZ_CP079181
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag KWY86_RS27565 Protein ID WP_001159868.1
Coordinates 53367..53672 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KWY86_RS27560 Protein ID WP_000813634.1
Coordinates 53147..53365 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS27530 (KWY86_27530) 48540..48956 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KWY86_RS27535 (KWY86_27535) 48953..49183 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
KWY86_RS27540 (KWY86_27540) 49448..49948 + 501 WP_000528931.1 HEPN family nuclease -
KWY86_RS27545 (KWY86_27545) 49961..50734 + 774 WP_000905949.1 hypothetical protein -
KWY86_RS27550 (KWY86_27550) 50901..52034 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
KWY86_RS27555 (KWY86_27555) 52068..52556 - 489 WP_011254646.1 hypothetical protein -
KWY86_RS27560 (KWY86_27560) 53147..53365 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KWY86_RS27565 (KWY86_27565) 53367..53672 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
KWY86_RS27570 (KWY86_27570) 53673..54479 + 807 WP_000016982.1 site-specific integrase -
KWY86_RS27575 (KWY86_27575) 55253..56008 + 756 WP_000852146.1 replication initiation protein RepE -
KWY86_RS27580 (KWY86_27580) 56596..57762 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..71307 71307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T209433 WP_001159868.1 NZ_CP079181:53367-53672 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T209433 NZ_CP104108:1720474-1720577 [Cronobacter sakazakii]
GGCAAGGCGACTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT209433 WP_000813634.1 NZ_CP079181:53147-53365 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT209433 NZ_CP104108:c1720580-1720412 [Cronobacter sakazakii]
GATAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAGTCGCCTTGCCTTTTAAGAATAGATGACGACACCAGCTTTTCCAGTTTGCGGCTAAAGTCGCAG
CAAAAAGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References