Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 48540..49183 Replicon plasmid pAI2040P_P2
Accession NZ_CP079181
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KWY86_RS27530 Protein ID WP_001034046.1
Coordinates 48540..48956 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KWY86_RS27535 Protein ID WP_001261278.1
Coordinates 48953..49183 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS27515 (KWY86_27515) 43677..44093 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KWY86_RS27520 (KWY86_27520) 44090..44320 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KWY86_RS27525 (KWY86_27525) 44701..48495 + 3795 WP_001144732.1 hypothetical protein -
KWY86_RS27530 (KWY86_27530) 48540..48956 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY86_RS27535 (KWY86_27535) 48953..49183 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KWY86_RS27540 (KWY86_27540) 49448..49948 + 501 WP_000528931.1 HEPN family nuclease -
KWY86_RS27545 (KWY86_27545) 49961..50734 + 774 WP_000905949.1 hypothetical protein -
KWY86_RS27550 (KWY86_27550) 50901..52034 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
KWY86_RS27555 (KWY86_27555) 52068..52556 - 489 WP_011254646.1 hypothetical protein -
KWY86_RS27560 (KWY86_27560) 53147..53365 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KWY86_RS27565 (KWY86_27565) 53367..53672 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..71307 71307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T209432 WP_001034046.1 NZ_CP079181:c48956-48540 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T209432 NZ_CP104108:1476935-1477297 [Cronobacter sakazakii]
ATGGCATGGACGGTGATTTTTACCGATCGTTTTAACGACTGGTATCAGCAGCAGCCCGAAGGATTACAGGATCGTATCGC
CGCGCTCTTATGGAACTTACGTTATTCCGGCCCGCTTGTCGGACGCCCGCTGGTCGATACGGTGAAAGGCTCGCGTTATC
CCAACCTGAAAGAGCTACGTGTGCAGTATGGCGGCGAACCCTGGCGCGTTTTTTTTGCGTTTGACGCTGAACGTCAGGCC
GTTGTGCTCTGCGCAGGCAATAAACGGAGCCAGAAACATTTTTATGACGCCTTAATCAGGCAGGCTGAAGAAGAATTCTT
CCTGCATGTGCAAGCGATGGAGAGACGAAATGAAAACTCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT209432 WP_001261278.1 NZ_CP079181:c49183-48953 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT209432 NZ_CP104108:1477284-1477580 [Cronobacter sakazakii]
ATGAAAACTCTTGATGACGTAATGGCGGGCTTTTCCCCGGAACGGCAGGCAGAAATCCTGCGCATGGCAGACGAAATCGC
GCTGGAGCACGGCCTGCCGCGCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAGCTGGCGGAGATAATGGGCGTGACGC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGTAAGGAGATTAAACTGCTGACGCTCAAGCGCTACGTCGAAGCGCTGGGC
GGCAAGCTCTCTTTACTGATAGAGCTGCCCGAAGGCAGCAAAGTGATCCCCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References