Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43677..44320 Replicon plasmid pAI2040P_P2
Accession NZ_CP079181
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KWY86_RS27515 Protein ID WP_001034044.1
Coordinates 43677..44093 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KWY86_RS27520 Protein ID WP_001261286.1
Coordinates 44090..44320 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS27500 (KWY86_27500) 40079..40776 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
KWY86_RS27505 (KWY86_27505) 41030..42052 - 1023 WP_000361402.1 helicase UvrD -
KWY86_RS27510 (KWY86_27510) 42037..43602 - 1566 WP_001128474.1 AAA family ATPase -
KWY86_RS27515 (KWY86_27515) 43677..44093 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY86_RS27520 (KWY86_27520) 44090..44320 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KWY86_RS27525 (KWY86_27525) 44701..48495 + 3795 WP_001144732.1 hypothetical protein -
KWY86_RS27530 (KWY86_27530) 48540..48956 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
KWY86_RS27535 (KWY86_27535) 48953..49183 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..71307 71307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T209431 WP_001034044.1 NZ_CP079181:c44093-43677 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T209431 NZ_CP104108:1414838-1415185 [Cronobacter sakazakii]
ATGTGGACTATTTACCTTACTCACGAGTTTGAGTGCTGGCTCGCGAGACAGCCGCAGCCGTTGCAGGAAGATGTGCTGGC
CGCGCTGGGGCTTTTGAAAATCAAAGGTCCGCACCTCGGGCGTCCGTATGCCGATACGCTCAAAGGCTCACGTCACACGG
GTATGAAGGCATTACGGGTTCAGCACGCCGTGCGGCCCATACGCGCCTTTTACGCTTTTGATCCGCACCGTTACGCGATT
GTGCTGTGCGCGGCACAGAAAAAGGGGGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACCGGGAGGTATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT209431 WP_001261286.1 NZ_CP079181:c44320-44090 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT209431 NZ_CP104108:1415186-1415485 [Cronobacter sakazakii]
ATGAAGACGTTTGATGAAGTGCTGGCGGCGCAGACCCCGGAGGCCCGCGCACGCATCGAAGAGAAAACGGCGCAACTGCT
GCTGGAAACCCACCTGTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGTATCGCAC
AGCCGTCAGTCGCGGCTATCGAACAACGCGGCAACGAGATGAAAATCTCAACGCTTAAGCGCTATGTCGAAGCCCTGGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References