Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5340311..5340936 Replicon chromosome
Accession NZ_CP079179
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY86_RS25990 Protein ID WP_002882817.1
Coordinates 5340311..5340694 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY86_RS25995 Protein ID WP_004150355.1
Coordinates 5340694..5340936 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS25975 (5337677) 5337677..5338579 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWY86_RS25980 (5338576) 5338576..5339211 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY86_RS25985 (5339208) 5339208..5340137 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KWY86_RS25990 (5340311) 5340311..5340694 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY86_RS25995 (5340694) 5340694..5340936 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY86_RS26000 (5341141) 5341141..5342058 + 918 WP_004181612.1 alpha/beta hydrolase -
KWY86_RS26005 (5342072) 5342072..5343013 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY86_RS26010 (5343058) 5343058..5343495 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KWY86_RS26015 (5343492) 5343492..5344352 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWY86_RS26020 (5344346) 5344346..5344945 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209430 WP_002882817.1 NZ_CP079179:c5340694-5340311 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209430 NZ_CP104108:c946114-945794 [Cronobacter sakazakii]
ATGCACATTTCATCTGCACTGATCCCGGTGCCGGTTGCGTCACGCCTGTCGCCTGTTCAGGTCTGGCAGCAACTCTTAAC
TTATCTTCTGGAACATCATTATGGTCTGGCACTTAACGACACGCCATTCCATGACGAAGCAGCTATTCAGGAACATATCG
AGGCGGGGATAACACTTGCTGATGCAGTGAATTTTCTTGTGGAGCGCTATGAACTTGTACGTACTGACCGCAAGGAATTC
ACATCGCAAGAGCAAACACCTTTTCTTACCGCCACCGATATTCTCATAGCCAAGAGAACTACCGGATTAATTCATACATA
A

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209430 WP_004150355.1 NZ_CP079179:c5340936-5340694 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209430 NZ_CP104108:c946482-946147 [Cronobacter sakazakii]
ATGGAGGATTTAATATCGTTAGTGCCATCCCATGACTGGGGACTACAAAGTGATATGATTTCACACTTTGGAGCAAGGCT
GGTTCAGGAAGACAACCGGCTACATTACCTAGCTGACAGGGCCGGGTTGACGGGAAGTTTTAGACCGGCGCAATTACAGA
AACTGGAACAAGCTTTCCCGCATTTTATGGAACAACTGGAAGTAATGCTGCGTTCCGGCGAGTTAAATCCACGCCAGCAA
CATCAGGCGCCCCTTCAGCTTGCAGATTTAACCTGCATAGCGGATACCCGTGGTAGCTGCGGCTACGTTTACATTTCTGT
TTATCCAACCCGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References