Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4722784..4723594 Replicon chromosome
Accession NZ_CP079179
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWY86_RS23110 Protein ID WP_004178461.1
Coordinates 4722784..4723317 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWY86_RS23115 Protein ID WP_002887278.1
Coordinates 4723328..4723594 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS23105 (4721615) 4721615..4722736 + 1122 WP_004214138.1 cupin domain-containing protein -
KWY86_RS23110 (4722784) 4722784..4723317 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWY86_RS23115 (4723328) 4723328..4723594 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWY86_RS23120 (4723697) 4723697..4725130 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
KWY86_RS23125 (4725120) 4725120..4725803 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWY86_RS23130 (4725975) 4725975..4727360 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
KWY86_RS23135 (4727378) 4727378..4727722 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209428 WP_004178461.1 NZ_CP079179:c4723317-4722784 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209428 NZ_CP104108:600092-600379 [Cronobacter sakazakii]
ATGAGCTATAAACTGGTCTTTGACCCACGTGCGCTGAAGGAGTGGCACAAACTGGGCGAGACCGTTAAAGCGCAATTCAA
AAAGAAGCTGGCGCACGTGCTGGCGGCTCCCAGGGTGAAATCCGCCCGGTTAAGTGGCCTGCCGGACTGCTACAAAATTA
AATTAAGAACGAGCGGTTACCGGCTGGTGTATCAGGTGCGCGATGATGCTGTCTGTGTGCTGGTCATCGCGATCGGCAAA
CGCGAAAACCTGACCGTATACCAGGGTGTCGGGCACCGTCTGGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209428 WP_002887278.1 NZ_CP079179:c4723594-4723328 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209428 NZ_CP104108:599860-600102 [Cronobacter sakazakii]
ATGGCCACAATCAACGTAAGGCTTGATGACAAGCTTAAAAATGAGGCTTATGCGGTGCTGGAAAAACTGGGTATCACACC
TACCGAAGCGGTGCGTCTGCTGTTTCAGTATGTGGCACAAAATGGCCGTATGCCGGTCAAAACGCTAACCGTCAGCGACA
GCGAAGAGGCGCTGCTGCGTACCGTGCGTGAGCGACTGGCGGACCCGCGGCCGGGCATCAGGGTTGATCTGGATGAGCTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References