Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4119293..4119912 Replicon chromosome
Accession NZ_CP079179
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWY86_RS20195 Protein ID WP_002892050.1
Coordinates 4119694..4119912 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWY86_RS20190 Protein ID WP_002892066.1
Coordinates 4119293..4119667 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY86_RS20180 (4114445) 4114445..4115638 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWY86_RS20185 (4115661) 4115661..4118807 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWY86_RS20190 (4119293) 4119293..4119667 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWY86_RS20195 (4119694) 4119694..4119912 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWY86_RS20200 (4120075) 4120075..4120641 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWY86_RS20205 (4120613) 4120613..4120753 - 141 WP_004147370.1 hypothetical protein -
KWY86_RS20210 (4120774) 4120774..4121244 + 471 WP_002892026.1 YlaC family protein -
KWY86_RS20215 (4121219) 4121219..4122670 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
KWY86_RS20220 (4122771) 4122771..4123469 + 699 WP_004177238.1 GNAT family protein -
KWY86_RS20225 (4123466) 4123466..4123606 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWY86_RS20230 (4123606) 4123606..4123869 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209427 WP_002892050.1 NZ_CP079179:4119694-4119912 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209427 NZ_CP104108:c217319-217044 [Cronobacter sakazakii]
ATGCTCCCCATTCGATGGACTGAAGAAGCAAAAATGGATCTTTTTGCGCTTATTAGCTTTATTGCCAGTGATAATCCAGG
TGCCGCGCAGGCGCTGTTAACGCGTATTGAAGAATCAATTTTACCCGCAGCGTACTATCCGGAGATGTTCCGCGAAGGCA
GAGTGCCAGATACGCGTGAAATCATTGCTCACCCGAATTACATCGTCGTTTATAAAGTCACTTCTGAATATCTACTGGTG
CTGAGCGTGCTGCATGCCCGTCAGCGCTACCCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209427 WP_002892066.1 NZ_CP079179:4119293-4119667 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209427 NZ_CP104108:c217510-217319 [Cronobacter sakazakii]
ATGCCAAAACATGACTCGCCCGGCGTCTCCAGTCGCAAGACCCATAAACAGGCGGAACAGTACGAGCTGTGGTTTCGTGA
AAAAGTGGAAGCCGCAGCGGCAAGCCGCCAACCCATTACGCCGCATGACGATGTGATGGCAAGCGCGCGCAAGATCATTG
AGAGCGCAAAAGTGAGGAGAAAAATGGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References