Detailed information of TA system TA209425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2782735..2784323
• Replicon: chromosome
• Accession: NZ_CP079179
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KWY86_RS13695
• Protein ID: WP_064162061.1
• Coordinates: 2782735..2784057 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: KWY86_RS13700
• Protein ID: WP_002904790.1
• Coordinates: 2784057..2784323 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY86_RS13680 (2777791)	2777791..2780292	+	2502	WP_218415635.1	fimbria/pilus outer membrane usher protein	-
KWY86_RS13685 (2780280)	2780280..2781284	+	1005	WP_064162060.1	fimbrial protein	-
KWY86_RS13690 (2781426)	2781426..2782718	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KWY86_RS13695 (2782735)	2782735..2784057	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
KWY86_RS13700 (2784057)	2784057..2784323	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KWY86_RS13705 (2784589)	2784589..2784915	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
KWY86_RS13710 (2784912)	2784912..2785412	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KWY86_RS13715 (2785390)	2785390..2786769	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
KWY86_RS13720 (2787115)	2787115..2788269	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KWY86_RS13725 (2788250)	2788250..2789173	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T209425 WP_064162061.1 NZ_CP079179:c2784057-2782735 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209425 NZ_CP104107:309599-309790 [Pseudomonas mosselii]
ATGAACAGCCGTGAAATGATTTCAGTGATCGAGGCGGATGGTTGGTACCTGGTGCGTTCGAAAGGCAGCCACCATCACTT
CAAGCATCCCGAAAAGGCAGGCCTGGTCACCATCCCGCATCCAAAGAAAGACCTGCTGCCGGCCACTGCGGCCAGCATCC
TGCGCCAGGCGCGGATCGGCTATGCCAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT209425 WP_002904790.1 NZ_CP079179:c2784323-2784057 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209425 NZ_CP104107:309808-310221 [Pseudomonas mosselii]
ATGTTGTTCCCCATTGCGATTCTCCCCGGTGACGATCAACACGCCTGGGGTGTGGAAGTCCCTGACATTCCGGGCTGTTT
CTCGGCCGGCGAGGACCTCGACAACGCCATTGCCATGGCCCGTGAAGCCATCGAAGGCCACCTCGAAATGCTGGCCCAGG
ACCAGCAGGAGATCCCCAAGGCCAGCCTGGTCAGCGTGCACGCGGCCAATCCTGCCTATGCCGGCTGTACCTGGGCGCTG
ATCGACATCGACATCACCCGTTACCTGGGCAAGGCCGAGAAGCTCAACATCACACTGCCGGCGTATTTGCTCACCCGCAT
CGACGCGTACGTGCAGAACCATCCGGAGCACAAGAGCCGTTCGGGCTTTTTGGCCGAAGCGGCGCTCAGGATCCTGCAGG
GGGACTGCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8