Detailed information of TA system TA209416

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 872645..873302
• Replicon: chromosome
• Accession: NZ_CP079179
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC13_AI2040PC

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KWY86_RS04365
• Protein ID: WP_040245400.1
• Coordinates: 872892..873302 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KWY86_RS04360
• Protein ID: WP_002916312.1
• Coordinates: 872645..872911 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY86_RS04335 (867801)	867801..869234	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
KWY86_RS04340 (869353)	869353..870081	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KWY86_RS04345 (870131)	870131..870442	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KWY86_RS04350 (870606)	870606..871265	+	660	WP_048334397.1	hemolysin III family protein	-
KWY86_RS04355 (871416)	871416..872399	-	984	WP_032430613.1	tRNA-modifying protein YgfZ	-
KWY86_RS04360 (872645)	872645..872911	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KWY86_RS04365 (872892)	872892..873302	+	411	WP_040245400.1	protein YgfX	Toxin
KWY86_RS04370 (873309)	873309..873830	-	522	WP_002916308.1	flavodoxin FldB	-
KWY86_RS04375 (873931)	873931..874827	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KWY86_RS04380 (874850)	874850..875563	+	714	WP_048290928.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KWY86_RS04385 (875569)	875569..877302	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16035.83 Da
• Isoelectric Point: 11.4778

>T209416 WP_040245400.1 NZ_CP079179:872892-873302 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDSRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T209416 NZ_CP104081:9386-9634 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT209416 WP_002916312.1 NZ_CP079179:872645-872911 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT209416 NZ_CP104081:9631-9978 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41