Detailed information of TA system TA209414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 170886..171411
• Replicon: plasmid pS471P_P1
• Accession: NZ_CP079173
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC11_S471PC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KWY79_RS27890
• Protein ID: WP_001159868.1
• Coordinates: 171106..171411 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KWY79_RS27885
• Protein ID: WP_000813634.1
• Coordinates: 170886..171104 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY79_RS27865 (KWY79_27865)	166824..168101	-	1278	WP_016338369.1	HlyD family secretion protein	-
KWY79_RS29365	168760..169296	+	537	WP_223825639.1	hypothetical protein	-
KWY79_RS29370	169314..169637	+	324	WP_085842301.1	hypothetical protein	-
KWY79_RS27875 (KWY79_27875)	169678..170094	-	417	WP_048983057.1	type II toxin-antitoxin system VapC family toxin	-
KWY79_RS27880 (KWY79_27880)	170091..170321	-	231	WP_016338373.1	type II toxin-antitoxin system VapB family antitoxin	-
KWY79_RS27885 (KWY79_27885)	170886..171104	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KWY79_RS27890 (KWY79_27890)	171106..171411	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KWY79_RS27895 (KWY79_27895)	171412..172218	+	807	WP_000016982.1	site-specific integrase	-
KWY79_RS27900 (KWY79_27900)	172992..173747	+	756	WP_000852146.1	replication initiation protein RepE	-
KWY79_RS27905 (KWY79_27905)	174335..175501	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / qnrB1 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr	-	1..210501	210501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T209414 WP_001159868.1 NZ_CP079173:171106-171411 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209414 NZ_CP104080:c18851-18435 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT209414 WP_000813634.1 NZ_CP079173:170886-171104 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209414 NZ_CP104080:c19078-18848 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2