Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5414916..5415541 Replicon chromosome
Accession NZ_CP079172
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC11_S471PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY79_RS26535 Protein ID WP_002882817.1
Coordinates 5414916..5415299 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY79_RS26540 Protein ID WP_004150355.1
Coordinates 5415299..5415541 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY79_RS26520 (5412282) 5412282..5413184 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWY79_RS26525 (5413181) 5413181..5413816 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY79_RS26530 (5413813) 5413813..5414742 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KWY79_RS26535 (5414916) 5414916..5415299 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY79_RS26540 (5415299) 5415299..5415541 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY79_RS26545 (5415746) 5415746..5416663 + 918 WP_004181612.1 alpha/beta hydrolase -
KWY79_RS26550 (5416677) 5416677..5417618 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY79_RS26555 (5417663) 5417663..5418100 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KWY79_RS26560 (5418097) 5418097..5418957 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWY79_RS26565 (5418951) 5418951..5419550 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209411 WP_002882817.1 NZ_CP079172:c5415299-5414916 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209411 NZ_CP104079:21164-21439 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGTCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTCAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209411 WP_004150355.1 NZ_CP079172:c5415541-5415299 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209411 NZ_CP104079:20880-21164 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTAATTACCGCACATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAAG
AGCGTAATCGCCTGACGCTGGAAGCCATGGACGATGTAACATCCGGACAGGTTATCAACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References