Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pCMC309P_P1
Accession NZ_CP079165
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC09_CMC309PC

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KWY74_RS25765 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KWY74_RS25760 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY74_RS25715 (KWY74_25715) 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KWY74_RS25720 (KWY74_25720) 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KWY74_RS25725 (KWY74_25725) 55851..56120 + 270 WP_004152102.1 hypothetical protein -
KWY74_RS25730 (KWY74_25730) 56108..56683 + 576 WP_004152103.1 hypothetical protein -
KWY74_RS25735 (KWY74_25735) 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
KWY74_RS25740 (KWY74_25740) 57252..57620 + 369 WP_004152105.1 hypothetical protein -
KWY74_RS25745 (KWY74_25745) 57654..57857 + 204 WP_004152106.1 HHA domain-containing protein -
KWY74_RS25750 (KWY74_25750) 57906..58163 + 258 WP_004152107.1 hypothetical protein -
KWY74_RS25755 (KWY74_25755) 58239..58493 + 255 WP_004152108.1 hypothetical protein -
KWY74_RS25760 (KWY74_25760) 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KWY74_RS25765 (KWY74_25765) 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KWY74_RS25770 (KWY74_25770) 59617..60963 + 1347 WP_020314316.1 ISNCY family transposase -
KWY74_RS27200 61124..61255 + 132 WP_256357619.1 hypothetical protein -
KWY74_RS25775 (KWY74_25775) 61464..62629 - 1166 Protein_67 IS3 family transposase -
KWY74_RS25780 (KWY74_25780) 62806..63768 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KWY74_RS25785 (KWY74_25785) 63755..64243 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..213013 213013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T209395 WP_003026803.1 NZ_CP079165:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T209395 NZ_CP104077:c2024965-2024858 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT209395 WP_003026799.1 NZ_CP079165:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209395 NZ_CP104077:2025012-2025078 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References