Detailed information of TA system TA209392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3369696..3370315
• Replicon: chromosome
• Accession: NZ_CP079164
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC09_CMC309PC

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: KWY74_RS16140
• Protein ID: WP_002892050.1
• Coordinates: 3369696..3369914 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: KWY74_RS16145
• Protein ID: WP_002892066.1
• Coordinates: 3369941..3370315 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY74_RS16105	3365743..3366006	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
KWY74_RS16110	3366006..3366146	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
KWY74_RS16115	3366143..3366841	-	699	WP_004178762.1	GNAT family protein	-
KWY74_RS16120	3366942..3368393	+	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
KWY74_RS16125	3368368..3368838	-	471	WP_002892026.1	YlaC family protein	-
KWY74_RS16130	3368859..3368999	+	141	WP_004147370.1	hypothetical protein	-
KWY74_RS16135	3368971..3369537	-	567	WP_002892030.1	maltose O-acetyltransferase	-
KWY74_RS16140	3369696..3369914	-	219	WP_002892050.1	HHA domain-containing protein	Toxin
KWY74_RS16145	3369941..3370315	-	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
KWY74_RS16150	3370801..3373947	-	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
KWY74_RS16155	3373970..3375163	-	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3364637..3365683	1046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T209392 WP_002892050.1 NZ_CP079164:c3369914-3369696 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T209392 NZ_CP104077:c1903323-1902829 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT209392 WP_002892066.1 NZ_CP079164:c3370315-3369941 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT209392 NZ_CP104077:c1903613-1903320 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A0H3GJ93