Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2193804..2194429 Replicon chromosome
Accession NZ_CP079164
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC09_CMC309PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY74_RS10625 Protein ID WP_002882817.1
Coordinates 2194046..2194429 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY74_RS10620 Protein ID WP_004150355.1
Coordinates 2193804..2194046 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY74_RS10595 (2189795) 2189795..2190394 + 600 WP_004151865.1 glucose-1-phosphatase -
KWY74_RS10600 (2190388) 2190388..2191248 + 861 WP_004146232.1 virulence factor BrkB family protein -
KWY74_RS10605 (2191245) 2191245..2191682 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY74_RS10610 (2191727) 2191727..2192668 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY74_RS10615 (2192682) 2192682..2193599 - 918 WP_004181612.1 alpha/beta hydrolase -
KWY74_RS10620 (2193804) 2193804..2194046 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY74_RS10625 (2194046) 2194046..2194429 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY74_RS10630 (2194603) 2194603..2195532 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY74_RS10635 (2195529) 2195529..2196164 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY74_RS10640 (2196161) 2196161..2197063 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209388 WP_002882817.1 NZ_CP079164:2194046-2194429 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209388 NZ_CP104077:c679269-678805 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209388 WP_004150355.1 NZ_CP079164:2193804-2194046 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209388 NZ_CP104077:c679604-679269 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References