Detailed information of TA system TA209387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1755434..1756020
• Replicon: chromosome
• Accession: NZ_CP079164
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC09_CMC309PC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KWY74_RS08600
• Protein ID: WP_002920800.1
• Coordinates: 1755434..1755802 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: KWY74_RS08605
• Protein ID: WP_004174006.1
• Coordinates: 1755799..1756020 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY74_RS08580 (1750937)	1750937..1752007	-	1071	WP_043906884.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
KWY74_RS08585 (1752009)	1752009..1752854	-	846	WP_004181473.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KWY74_RS08590 (1752851)	1752851..1753738	-	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KWY74_RS08595 (1753845)	1753845..1755161	-	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KWY74_RS08600 (1755434)	1755434..1755802	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KWY74_RS08605 (1755799)	1755799..1756020	-	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KWY74_RS08610 (1756184)	1756184..1756897	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KWY74_RS08615 (1756899)	1756899..1757666	-	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KWY74_RS08620 (1757663)	1757663..1758940	-	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
KWY74_RS08625 (1758937)	1758937..1759863	-	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1750200..1795780	45580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T209387 WP_002920800.1 NZ_CP079164:c1755802-1755434 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T209387 NZ_CP104077:351288-351563 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT209387 WP_004174006.1 NZ_CP079164:c1756020-1755799 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT209387 NZ_CP104077:351560-351919 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7