Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /RES-TIGR02293 |
| Location | 167490..168400 | Replicon | plasmid pCMC432M_P1 |
| Accession | NZ_CP079156 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC08_CMC432MC | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | A0A5Q9LR54 |
| Locus tag | KWY73_RS26250 | Protein ID | WP_004181896.1 |
| Coordinates | 167930..168400 (+) | Length | 157 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | A0A2J4RLY9 |
| Locus tag | KWY73_RS26245 | Protein ID | WP_004181895.1 |
| Coordinates | 167490..167933 (+) | Length | 148 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY73_RS26225 (KWY73_26225) | 162767..163474 | + | 708 | WP_062826779.1 | hypothetical protein | - |
| KWY73_RS26230 (KWY73_26230) | 163479..164510 | + | 1032 | WP_170856552.1 | CRISPR-associated protein Csf2 | - |
| KWY73_RS26235 (KWY73_26235) | 165696..166118 | + | 423 | WP_004181893.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| KWY73_RS26240 (KWY73_26240) | 166118..167389 | + | 1272 | WP_004181894.1 | translesion error-prone DNA polymerase V subunit UmuC | - |
| KWY73_RS26245 (KWY73_26245) | 167490..167933 | + | 444 | WP_004181895.1 | DUF2384 domain-containing protein | Antitoxin |
| KWY73_RS26250 (KWY73_26250) | 167930..168400 | + | 471 | WP_004181896.1 | RES family NAD+ phosphorylase | Toxin |
| KWY73_RS26255 (KWY73_26255) | 168510..168770 | - | 261 | WP_004026352.1 | hypothetical protein | - |
| KWY73_RS26260 (KWY73_26260) | 169341..170522 | - | 1182 | WP_004883380.1 | IS481 family transposase | - |
| KWY73_RS26265 (KWY73_26265) | 170594..170752 | + | 159 | WP_004181898.1 | type I toxin-antitoxin system Hok family toxin | - |
| KWY73_RS26270 (KWY73_26270) | 171355..172329 | + | 975 | WP_043907013.1 | hypothetical protein | - |
| KWY73_RS26275 (KWY73_26275) | 172527..172904 | - | 378 | WP_004181901.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..285823 | 285823 | |
| - | flank | IS/Tn | - | - | 169341..170522 | 1181 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 157 a.a. Molecular weight: 17549.81 Da Isoelectric Point: 4.6155
>T209375 WP_004181896.1 NZ_CP079156:167930-168400 [Klebsiella pneumoniae subsp. pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
Download Length: 471 bp
>T209375 NZ_CP104049:4194294-4194605 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
Antitoxin
Download Length: 148 a.a. Molecular weight: 16536.87 Da Isoelectric Point: 10.3013
>AT209375 WP_004181895.1 NZ_CP079156:167490-167933 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
Download Length: 444 bp
>AT209375 NZ_CP104049:4194606-4194896 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Q9LR54 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4RLY9 |