Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | stbDE/ParE-YafN |
| Location | 23055..23577 | Replicon | plasmid pCMC432M_P1 |
| Accession | NZ_CP079156 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC08_CMC432MC | ||
Toxin (Protein)
| Gene name | stbE | Uniprot ID | A0A0C7KG00 |
| Locus tag | KWY73_RS25475 | Protein ID | WP_038991638.1 |
| Coordinates | 23293..23577 (+) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | stbD | Uniprot ID | A0A2J4R0U8 |
| Locus tag | KWY73_RS25470 | Protein ID | WP_004181777.1 |
| Coordinates | 23055..23303 (+) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY73_RS25450 (KWY73_25450) | 18264..19085 | - | 822 | WP_004181772.1 | hypothetical protein | - |
| KWY73_RS25455 (KWY73_25455) | 19147..19500 | - | 354 | WP_004181774.1 | hypothetical protein | - |
| KWY73_RS25460 (KWY73_25460) | 19645..20631 | - | 987 | WP_040120328.1 | hypothetical protein | - |
| KWY73_RS25465 (KWY73_25465) | 20965..22764 | - | 1800 | WP_043907032.1 | ATP-dependent helicase | - |
| KWY73_RS25470 (KWY73_25470) | 23055..23303 | + | 249 | WP_004181777.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| KWY73_RS25475 (KWY73_25475) | 23293..23577 | + | 285 | WP_038991638.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KWY73_RS25480 (KWY73_25480) | 23594..23695 | - | 102 | Protein_27 | IS200/IS605 family transposase | - |
| KWY73_RS25485 (KWY73_25485) | 23731..24957 | + | 1227 | WP_040120327.1 | RNA-guided endonuclease TnpB family protein | - |
| KWY73_RS25490 (KWY73_25490) | 25229..25453 | - | 225 | Protein_29 | transposase | - |
| KWY73_RS25495 (KWY73_25495) | 25532..25960 | - | 429 | WP_077256229.1 | IS200/IS605 family transposase | - |
| KWY73_RS25500 (KWY73_25500) | 25996..27183 | + | 1188 | WP_043907031.1 | RNA-guided endonuclease TnpB family protein | - |
| KWY73_RS29180 | 27228..27599 | - | 372 | WP_044816264.1 | hypothetical protein | - |
| KWY73_RS29185 | 27596..27940 | - | 345 | WP_014386518.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..285823 | 285823 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10993.73 Da Isoelectric Point: 10.6516
>T209374 WP_038991638.1 NZ_CP079156:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN
Download Length: 285 bp
>T209374 NZ_CP104049:c4193061-4192750 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 8925.17 Da Isoelectric Point: 3.8209
>AT209374 WP_004181777.1 NZ_CP079156:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL
Download Length: 249 bp
>AT209374 NZ_CP104049:c4192753-4192307 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0C7KG00 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4R0U8 |