Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1620096..1620622 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag S_RS08820 Protein ID WP_000323025.1
Coordinates 1620096..1620383 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag S_RS08825 Protein ID WP_000534858.1
Coordinates 1620383..1620622 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS08760 1615154..1615489 + 336 WP_000871291.1 anti-adapter protein IraM -
S_RS27675 1615770..1615878 - 109 Protein_1601 DUF3927 family protein -
S_RS08790 1616799..1617620 - 822 WP_000762882.1 antitermination protein -
S_RS08795 1617635..1617991 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
S_RS08800 1618004..1619053 - 1050 WP_001265249.1 DUF968 domain-containing protein -
S_RS08805 1619055..1619333 - 279 Protein_1605 hypothetical protein -
S_RS08810 1619400..1619651 - 252 WP_000980987.1 hypothetical protein -
S_RS08815 1619869..1620024 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
S_RS08820 1620096..1620383 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
S_RS08825 1620383..1620622 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
S_RS25680 1621926..1622204 + 279 WP_014532248.1 hypothetical protein -
S_RS08850 1623419..1624438 + 1020 Protein_1613 MHS family MFS transporter YdfJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1604045..1632392 28347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T20937 WP_000323025.1 NC_004741:c1620383-1620096 [Shigella flexneri 2a str. 2457T]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T20937 NC_004741:c1620383-1620096 [Shigella flexneri 2a str. 2457T]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT20937 WP_000534858.1 NC_004741:c1620622-1620383 [Shigella flexneri 2a str. 2457T]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT20937 NC_004741:c1620622-1620383 [Shigella flexneri 2a str. 2457T]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References