Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2259467..2260092 Replicon chromosome
Accession NZ_CP079155
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC08_CMC432MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY73_RS11005 Protein ID WP_002882817.1
Coordinates 2259709..2260092 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY73_RS11000 Protein ID WP_004150355.1
Coordinates 2259467..2259709 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY73_RS10975 (2255458) 2255458..2256057 + 600 WP_004151865.1 glucose-1-phosphatase -
KWY73_RS10980 (2256051) 2256051..2256911 + 861 WP_004146232.1 virulence factor BrkB family protein -
KWY73_RS10985 (2256908) 2256908..2257345 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY73_RS10990 (2257390) 2257390..2258331 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY73_RS10995 (2258345) 2258345..2259262 - 918 WP_004181612.1 alpha/beta hydrolase -
KWY73_RS11000 (2259467) 2259467..2259709 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY73_RS11005 (2259709) 2259709..2260092 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY73_RS11010 (2260266) 2260266..2261195 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY73_RS11015 (2261192) 2261192..2261827 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY73_RS11020 (2261824) 2261824..2262726 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209367 WP_002882817.1 NZ_CP079155:2259709-2260092 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209367 NZ_CP104049:c734882-734625 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209367 WP_004150355.1 NZ_CP079155:2259467-2259709 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209367 NZ_CP104049:734707-735039 [Salmonella enterica subsp. enterica serovar Kentucky]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGG
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTTAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References