Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /RES-TIGR02293 |
| Location | 167689..168599 | Replicon | plasmid pCMC432P_P1 |
| Accession | NZ_CP079151 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | A0A5Q9LR54 |
| Locus tag | KWY72_RS27270 | Protein ID | WP_004181896.1 |
| Coordinates | 168129..168599 (+) | Length | 157 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | A0A2J4RLY9 |
| Locus tag | KWY72_RS27265 | Protein ID | WP_004181895.1 |
| Coordinates | 167689..168132 (+) | Length | 148 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY72_RS27245 (KWY72_27245) | 162966..163673 | + | 708 | WP_062826779.1 | hypothetical protein | - |
| KWY72_RS27250 (KWY72_27250) | 163678..164709 | + | 1032 | WP_170856552.1 | CRISPR-associated protein Csf2 | - |
| KWY72_RS27255 (KWY72_27255) | 165895..166317 | + | 423 | WP_004181893.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| KWY72_RS27260 (KWY72_27260) | 166317..167588 | + | 1272 | WP_004181894.1 | translesion error-prone DNA polymerase V subunit UmuC | - |
| KWY72_RS27265 (KWY72_27265) | 167689..168132 | + | 444 | WP_004181895.1 | DUF2384 domain-containing protein | Antitoxin |
| KWY72_RS27270 (KWY72_27270) | 168129..168599 | + | 471 | WP_004181896.1 | RES family NAD+ phosphorylase | Toxin |
| KWY72_RS27275 (KWY72_27275) | 168709..168969 | - | 261 | WP_004026352.1 | hypothetical protein | - |
| KWY72_RS27280 (KWY72_27280) | 169540..170721 | - | 1182 | WP_004883380.1 | IS481 family transposase | - |
| KWY72_RS27285 (KWY72_27285) | 170793..170951 | + | 159 | WP_004181898.1 | type I toxin-antitoxin system Hok family toxin | - |
| KWY72_RS27290 (KWY72_27290) | 171554..172528 | + | 975 | WP_043907013.1 | hypothetical protein | - |
| KWY72_RS27295 (KWY72_27295) | 172726..173103 | - | 378 | WP_004181901.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..289419 | 289419 | |
| - | flank | IS/Tn | - | - | 169540..170721 | 1181 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 157 a.a. Molecular weight: 17549.81 Da Isoelectric Point: 4.6155
>T209354 WP_004181896.1 NZ_CP079151:168129-168599 [Klebsiella pneumoniae subsp. pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK
Download Length: 471 bp
>T209354 NZ_CP104042:c441509-441216 [Rhizobium leguminosarum bv. viciae]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGCAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCAGG
CACGGCAAAGCGATACCACGATGAACTTTTCGCGTTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCTGTTAGAATTCATCCCTTCAAAGCGCATCTAGTCGTTTATCGGATCGAAGATGATGAGACAATT
TTCGTAATACGAATTCGCCACGCCCATGAAGATTGGGCAACTGATTCAATTTAG
ATGAGTTTTAAGCTTTCTGCCGAAGCGGCAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCAGG
CACGGCAAAGCGATACCACGATGAACTTTTCGCGTTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCTGTTAGAATTCATCCCTTCAAAGCGCATCTAGTCGTTTATCGGATCGAAGATGATGAGACAATT
TTCGTAATACGAATTCGCCACGCCCATGAAGATTGGGCAACTGATTCAATTTAG
Antitoxin
Download Length: 148 a.a. Molecular weight: 16536.87 Da Isoelectric Point: 10.3013
>AT209354 WP_004181895.1 NZ_CP079151:167689-168132 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS
Download Length: 444 bp
>AT209354 NZ_CP104042:c441748-441506 [Rhizobium leguminosarum bv. viciae]
ATGGCCACGATGAACGTATCTCTTCCACGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAGGACCGGGCGATATTCCAA
TGCTAGCGACTACGTGCGTGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTTTAGGCATCCAATCAAAGGATGAACTCTTTGCTGAAGCGATAGCTCGTGCCAGCAAGTCA
TGA
ATGGCCACGATGAACGTATCTCTTCCACGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAGGACCGGGCGATATTCCAA
TGCTAGCGACTACGTGCGTGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTTTAGGCATCCAATCAAAGGATGAACTCTTTGCTGAAGCGATAGCTCGTGCCAGCAAGTCA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Q9LR54 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4RLY9 |