209353

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 23055..23577 Replicon plasmid pCMC432P_P1
Accession NZ_CP079151
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag KWY72_RS26495 Protein ID WP_038991638.1
Coordinates 23293..23577 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag KWY72_RS26490 Protein ID WP_004181777.1
Coordinates 23055..23303 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY72_RS26470 (KWY72_26470) 18264..19085 - 822 WP_004181772.1 hypothetical protein -
KWY72_RS26475 (KWY72_26475) 19147..19500 - 354 WP_004181774.1 hypothetical protein -
KWY72_RS26480 (KWY72_26480) 19645..20631 - 987 WP_040120328.1 hypothetical protein -
KWY72_RS26485 (KWY72_26485) 20965..22764 - 1800 WP_043907032.1 ATP-dependent helicase -
KWY72_RS26490 (KWY72_26490) 23055..23303 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KWY72_RS26495 (KWY72_26495) 23293..23577 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KWY72_RS26500 (KWY72_26500) 23594..23695 - 102 Protein_27 IS200/IS605 family transposase -
KWY72_RS26505 (KWY72_26505) 23731..24957 + 1227 WP_040120327.1 RNA-guided endonuclease TnpB family protein -
KWY72_RS26510 (KWY72_26510) 25229..25453 - 225 Protein_29 transposase -
KWY72_RS26515 (KWY72_26515) 25532..25960 - 429 WP_077256229.1 IS200/IS605 family transposase -
KWY72_RS26520 (KWY72_26520) 25996..27183 + 1188 WP_043907031.1 RNA-guided endonuclease TnpB family protein -
KWY72_RS29230 27228..27599 - 372 WP_044816264.1 hypothetical protein -
KWY72_RS29235 27596..27940 - 345 WP_014386518.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..289419 289419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-84)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T209353 WP_038991638.1 NZ_CP079151:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T209353 NZ_CP104042:96924-97214 [Rhizobium leguminosarum bv. viciae]
ATGACCGACATCATATTTTCTCCCGCTGCACAAACCGATATTGACAAGATTTGGGATTACACAGCGACGAGTTGGAATGT
CGATCAGGCAGAGCGTTATATTCAGGACATAAGAGACGCCTGTCAGGATCTGGCGGAGGGAACACGGATGAGCCGCCCCT
CAGATATTCGGAAAGGGTACAGAAAGGTCTCCGTTGGCTCTCATTTTCTGTATTTCCAAAGCAATGATGCGGGCCAGATC
ATCATCGTGCGCATCCTTCATCAACGAATGGACGTAGCCAAGCACCTGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT209353 WP_004181777.1 NZ_CP079151:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT209353 NZ_CP104042:96679-96927 [Rhizobium leguminosarum bv. viciae]
ATGGCCAGAAATACGTCCATATCGCTCGGCGAGCATTTCACCACCTTCATCGATACGCAAGTTCAGGCTGGCCGCTATGG
TTCAGCCACTGACGTTGTTCGGGCCGGCCTTCGCCTCCTTGAAGAACACGAAGCCAAAGTGAAGGCATTACAGGATGCCT
TGATTGAAGGCGAGGAATCCGGCCCTGCCACGCCCTTTGATTTCGATGCGTTCAACGCTCGCAAACGGGCGGCGTTCAAG
GCGAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00


Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References