Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5363627..5364252 Replicon chromosome
Accession NZ_CP079150
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY72_RS25915 Protein ID WP_002882817.1
Coordinates 5363627..5364010 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY72_RS25920 Protein ID WP_004150355.1
Coordinates 5364010..5364252 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY72_RS25900 (5360993) 5360993..5361895 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWY72_RS25905 (5361892) 5361892..5362527 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY72_RS25910 (5362524) 5362524..5363453 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY72_RS25915 (5363627) 5363627..5364010 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY72_RS25920 (5364010) 5364010..5364252 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY72_RS25925 (5364457) 5364457..5365374 + 918 WP_004181612.1 alpha/beta hydrolase -
KWY72_RS25930 (5365388) 5365388..5366329 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY72_RS25935 (5366374) 5366374..5366811 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY72_RS25940 (5366808) 5366808..5367668 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWY72_RS25945 (5367662) 5367662..5368261 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209352 WP_002882817.1 NZ_CP079150:c5364010-5363627 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209352 NZ_CP104041:298090-298617 [Rhizobium leguminosarum bv. viciae]
GTGACCCTCCCCGTCTGGCATGAAGAGCCGATCGCTAAGTCGCACGATCGGGCCGCATTCGATTGCGGTGATGCGGCAAT
GAACGAGTTCATTCGGCGCTTCGCTCGGCAGAGCCATGAGCAGAATGCGGCGAAGACCTTCTGCGCTATCGACAAGGCCC
AACCTGATTGCATCCTCGGATTCTACACAGTCGCGCCTTCGGCCGTTACGCATGAGGCCGTGCCTCCGAAGATGACGAGA
GGCCTCGCGCGCCATGAAGTCGCAGGCTTCAAGCTCGCGCGCCTGGCAACCGACATAAAGGTGGCGGGAAAAGGTTTAGG
CGGCCAGCTCATCGCCGCTGCGGCGCTCCGTTGCCTGCGGCTCGCCAGCGAGGGCGGCGGGATCCTGCTTATCATCGATG
CCAAGAGCGAACGCGCCGCCCATTGGTATGCCGGCTACGGCGCCGAACCGCTGCAAGGGAAACCGCTCACCCTCGTTATG
CCGCTTGCCACCTTCGCCGCCGACTTCAAGGCCAAGGGGCTCTTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209352 WP_004150355.1 NZ_CP079150:c5364252-5364010 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209352 NZ_CP104041:297815-298093 [Rhizobium leguminosarum bv. viciae]
ATGCCGAGAACCGCGGACAGTAAAACCGAACGCTACCAGCTGCGCATCCCGCCAAAGCAGAAGGCAATGATCGCGCGCGC
GGCTGCCCTCTCGAACAAGGATATGGCCGATTTCATTCTCGATAAGATCGTACCTGAAGCCGAAGCCGTCATCCAGGCTG
CGGAAGTTGAGGCCGTTTCGAGCCGCGATTTCATCCGCATCCTCGATCTGCTTGAACACCCGCCCAAACCGAACGCGAAA
CTACGCGCCGCGATCTCTGCTCTACCGGATGCCTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References