Detailed information of TA system TA209352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5363627..5364252
• Replicon: chromosome
• Accession: NZ_CP079150
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: KWY72_RS25915
• Protein ID: WP_002882817.1
• Coordinates: 5363627..5364010 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: KWY72_RS25920
• Protein ID: WP_004150355.1
• Coordinates: 5364010..5364252 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY72_RS25900 (5360993)	5360993..5361895	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
KWY72_RS25905 (5361892)	5361892..5362527	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
KWY72_RS25910 (5362524)	5362524..5363453	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
KWY72_RS25915 (5363627)	5363627..5364010	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
KWY72_RS25920 (5364010)	5364010..5364252	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
KWY72_RS25925 (5364457)	5364457..5365374	+	918	WP_004181612.1	alpha/beta hydrolase	-
KWY72_RS25930 (5365388)	5365388..5366329	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
KWY72_RS25935 (5366374)	5366374..5366811	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
KWY72_RS25940 (5366808)	5366808..5367668	-	861	WP_004146232.1	virulence factor BrkB family protein	-
KWY72_RS25945 (5367662)	5367662..5368261	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T209352 WP_002882817.1 NZ_CP079150:c5364010-5363627 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T209352 NZ_CP104041:298090-298617 [Rhizobium leguminosarum bv. viciae]
GTGACCCTCCCCGTCTGGCATGAAGAGCCGATCGCTAAGTCGCACGATCGGGCCGCATTCGATTGCGGTGATGCGGCAAT
GAACGAGTTCATTCGGCGCTTCGCTCGGCAGAGCCATGAGCAGAATGCGGCGAAGACCTTCTGCGCTATCGACAAGGCCC
AACCTGATTGCATCCTCGGATTCTACACAGTCGCGCCTTCGGCCGTTACGCATGAGGCCGTGCCTCCGAAGATGACGAGA
GGCCTCGCGCGCCATGAAGTCGCAGGCTTCAAGCTCGCGCGCCTGGCAACCGACATAAAGGTGGCGGGAAAAGGTTTAGG
CGGCCAGCTCATCGCCGCTGCGGCGCTCCGTTGCCTGCGGCTCGCCAGCGAGGGCGGCGGGATCCTGCTTATCATCGATG
CCAAGAGCGAACGCGCCGCCCATTGGTATGCCGGCTACGGCGCCGAACCGCTGCAAGGGAAACCGCTCACCCTCGTTATG
CCGCTTGCCACCTTCGCCGCCGACTTCAAGGCCAAGGGGCTCTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT209352 WP_004150355.1 NZ_CP079150:c5364252-5364010 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT209352 NZ_CP104041:297815-298093 [Rhizobium leguminosarum bv. viciae]
ATGCCGAGAACCGCGGACAGTAAAACCGAACGCTACCAGCTGCGCATCCCGCCAAAGCAGAAGGCAATGATCGCGCGCGC
GGCTGCCCTCTCGAACAAGGATATGGCCGATTTCATTCTCGATAAGATCGTACCTGAAGCCGAAGCCGTCATCCAGGCTG
CGGAAGTTGAGGCCGTTTCGAGCCGCGATTTCATCCGCATCCTCGATCTGCTTGAACACCCGCCCAAACCGAACGCGAAA
CTACGCGCCGCGATCTCTGCTCTACCGGATGCCTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9