20935

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 378572..379416 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag S_RS01965 Protein ID WP_005060796.1
Coordinates 378572..378970 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag S_RS01975 Protein ID WP_005053053.1
Coordinates 379105..379416 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS01945 373741..375471 - 1731 WP_000645013.1 hypothetical protein -
S_RS01950 375524..377677 - 2154 WP_005060811.1 SEL1-like repeat protein -
S_RS24935 377828..378524 + 697 Protein_355 IS1 family transposase -
S_RS01965 378572..378970 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
S_RS01975 379105..379416 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
S_RS01980 379600..380490 - 891 WP_000971346.1 heme o synthase -
S_RS01985 380502..380831 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
S_RS01990 380831..381445 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
S_RS01995 381435..383426 - 1992 Protein_361 cytochrome o ubiquinol oxidase subunit I -
S_RS02000 383448..383945 - 498 Protein_362 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T20935 WP_005060796.1 NC_004741:c378970-378572 [Shigella flexneri 2a str. 2457T]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T20935 NC_004741:c378970-378572 [Shigella flexneri 2a str. 2457T]
GTGCGTAATAGCGACTGTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAGACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCA
TGTTGGGGGGAGCGCGGAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTATTATGTGATTTTTTTGAACATGTAAAAAAA
ATTCACCCGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTT
TGTTCAGCTTTCAGCGACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT20935 WP_005053053.1 NC_004741:c379416-379105 [Shigella flexneri 2a str. 2457T]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT20935 NC_004741:c379416-379105 [Shigella flexneri 2a str. 2457T]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References