Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4784605..4785415 Replicon chromosome
Accession NZ_CP079150
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWY72_RS23240 Protein ID WP_004178461.1
Coordinates 4784605..4785138 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWY72_RS23245 Protein ID WP_002887278.1
Coordinates 4785149..4785415 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY72_RS23235 (4783436) 4783436..4784557 + 1122 WP_002887282.1 cupin domain-containing protein -
KWY72_RS23240 (4784605) 4784605..4785138 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWY72_RS23245 (4785149) 4785149..4785415 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWY72_RS23250 (4785518) 4785518..4786951 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KWY72_RS23255 (4786941) 4786941..4787624 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWY72_RS23260 (4787796) 4787796..4789181 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KWY72_RS23265 (4789199) 4789199..4789543 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209349 WP_004178461.1 NZ_CP079150:c4785138-4784605 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209349 NZ_CP104039:c116265-115972 [Rhizobium leguminosarum bv. viciae]
ATGAGTTTTAGGCTTTCTGCCGAAGCGGAAGAGGACATCATTGCGATCGCCGAGCAAGGTGTGCGTCTGTTCGGGGCAGG
CCAAGCAAAGCGATACCACGATGAACTTTTCGCATTATTTGATCTGATAGCTGCCAATCCACGCATCGCGCGCGAACGGG
ATGTGATCGATCCGCCCGTTAGAATTCATCCCTTCAAAGCGCATCTAATCGTTTATCGGATCGAAAATGATGAAACAATT
TTTGTAATACGAATTCGCCACGCGCATGAAGATTGGGCAACTGATTCAATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209349 WP_002887278.1 NZ_CP079150:c4785415-4785149 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209349 NZ_CP104039:c116504-116262 [Rhizobium leguminosarum bv. viciae]
ATGGCCACGATGAACGTATCTCTTCCGGGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAAGACCGGGCGATATTCGAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAAACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGCGGTGTCGGCGTTCGATCAAAGGATGAACTCTTTGCTGAGGCGATGGCTCGTACCAGCAAGTCA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References