Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4190029..4190648 Replicon chromosome
Accession NZ_CP079150
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC07_CMC432PC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWY72_RS20415 Protein ID WP_002892050.1
Coordinates 4190430..4190648 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWY72_RS20410 Protein ID WP_002892066.1
Coordinates 4190029..4190403 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY72_RS20400 (4185181) 4185181..4186374 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWY72_RS20405 (4186397) 4186397..4189543 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWY72_RS20410 (4190029) 4190029..4190403 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWY72_RS20415 (4190430) 4190430..4190648 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWY72_RS20420 (4190807) 4190807..4191373 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWY72_RS20425 (4191345) 4191345..4191485 - 141 WP_004147370.1 hypothetical protein -
KWY72_RS20430 (4191506) 4191506..4191976 + 471 WP_002892026.1 YlaC family protein -
KWY72_RS20435 (4191951) 4191951..4193402 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KWY72_RS20440 (4193503) 4193503..4194201 + 699 WP_004178762.1 GNAT family protein -
KWY72_RS20445 (4194198) 4194198..4194338 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWY72_RS20450 (4194338) 4194338..4194601 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209348 WP_002892050.1 NZ_CP079150:4190430-4190648 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209348 NZ_CP104038:61746-62018 [Rhizobium leguminosarum bv. viciae]
ATGAAATTGCTATGGACGCCGAATTCCTGGGAAGAGTACGAATACTGGCAGAAAAGCGACCAGAAGATGGTCGAGAAAAT
CAACGAGCTCCTGAAAGATACGAAAAGGTCACCCTTTAAAGTTCTTGACAAGCCAGAGCCATTGAAGGGCGATCTATCCG
GATTTTGGTCAAGGCGCATCCTCGGAGAGCATCGGCTCGTCTACTGCGTGACTGGCAAGGGTTCCGACCAGCAGTTGGAA
ATCATTCAGTGCCGATTTCATTACGAAAAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209348 WP_002892066.1 NZ_CP079150:4190029-4190403 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209348 NZ_CP104038:61493-61744 [Rhizobium leguminosarum bv. viciae]
ATGGCCCACGTTCGTTTGACGGAGTTCCGGCAAAATATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCTCT
GCTCGTGACCCGTCAAGGTTCGGAAGCAATGGTCTTGGTTGCCGAAGGCGAATACGAGAGTATGCAAGAGATGTTGCACC
TGCTCTCGACGCCTGCTAATTCTGCCCGGCTACGCGAAAGTATCGGACAGCTTCGCAAGGGGCAGGTGGTAGAGAGCGAT
CCCACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References