Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ataRT/ElaA-DUF1778 |
| Location | 245089..245840 | Replicon | plasmid pCMC387M_P1 |
| Accession | NZ_CP079146 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC | ||
Toxin (Protein)
| Gene name | ataT | Uniprot ID | H6U1U8 |
| Locus tag | KWY71_RS27655 | Protein ID | WP_014386536.1 |
| Coordinates | 245089..245571 (-) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | ataR | Uniprot ID | A0A071LPN3 |
| Locus tag | KWY71_RS27660 | Protein ID | WP_004902250.1 |
| Coordinates | 245562..245840 (-) | Length | 93 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY71_RS27635 (KWY71_27635) | 241488..242135 | - | 648 | WP_014386537.1 | EcsC family protein | - |
| KWY71_RS27640 (KWY71_27640) | 242162..242917 | - | 756 | WP_004902235.1 | DUF2971 domain-containing protein | - |
| KWY71_RS27645 (KWY71_27645) | 243018..243410 | - | 393 | WP_032442757.1 | hypothetical protein | - |
| KWY71_RS27650 (KWY71_27650) | 243515..244054 | - | 540 | WP_004902239.1 | hypothetical protein | - |
| KWY71_RS27655 (KWY71_27655) | 245089..245571 | - | 483 | WP_014386536.1 | GNAT family N-acetyltransferase | Toxin |
| KWY71_RS27660 (KWY71_27660) | 245562..245840 | - | 279 | WP_004902250.1 | DUF1778 domain-containing protein | Antitoxin |
| KWY71_RS27665 (KWY71_27665) | 245959..246171 | - | 213 | WP_004902255.1 | hypothetical protein | - |
| KWY71_RS27670 (KWY71_27670) | 246279..246620 | - | 342 | WP_004902257.1 | hypothetical protein | - |
| KWY71_RS27675 (KWY71_27675) | 247450..247908 | - | 459 | WP_014386535.1 | hypothetical protein | - |
| KWY71_RS27680 (KWY71_27680) | 248561..249016 | - | 456 | WP_001166628.1 | Hg(II)-responsive transcriptional regulator | - |
| KWY71_RS27685 (KWY71_27685) | 249088..249453 | + | 366 | WP_004200999.1 | mercuric ion transporter MerT | - |
| KWY71_RS27690 (KWY71_27690) | 249469..249744 | + | 276 | WP_000732275.1 | mercury resistance system periplasmic binding protein MerP | - |
| KWY71_RS27695 (KWY71_27695) | 249772..250197 | + | 426 | WP_000522996.1 | organomercurial transporter MerC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..286022 | 286022 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17662.45 Da Isoelectric Point: 8.9130
>T209334 WP_014386536.1 NZ_CP079146:c245571-245089 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
Download Length: 483 bp
>T209334 NZ_CP104030:c5375551-5375168 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
Antitoxin
Download Length: 93 a.a. Molecular weight: 10704.23 Da Isoelectric Point: 4.5271
>AT209334 WP_004902250.1 NZ_CP079146:c245840-245562 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
Download Length: 279 bp
>AT209334 NZ_CP104030:c5375793-5375551 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2A5MBI1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A071LPN3 |