Detailed information of TA system TA209331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5363617..5364242
• Replicon: chromosome
• Accession: NZ_CP079145
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: KWY71_RS25915
• Protein ID: WP_002882817.1
• Coordinates: 5363617..5364000 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: KWY71_RS25920
• Protein ID: WP_004150355.1
• Coordinates: 5364000..5364242 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY71_RS25900 (5360983)	5360983..5361885	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
KWY71_RS25905 (5361882)	5361882..5362517	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
KWY71_RS25910 (5362514)	5362514..5363443	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
KWY71_RS25915 (5363617)	5363617..5364000	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
KWY71_RS25920 (5364000)	5364000..5364242	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
KWY71_RS25925 (5364447)	5364447..5365364	+	918	WP_004181612.1	alpha/beta hydrolase	-
KWY71_RS25930 (5365378)	5365378..5366319	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
KWY71_RS25935 (5366364)	5366364..5366801	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
KWY71_RS25940 (5366798)	5366798..5367658	-	861	WP_004146232.1	virulence factor BrkB family protein	-
KWY71_RS25945 (5367652)	5367652..5368251	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T209331 WP_002882817.1 NZ_CP079145:c5364000-5363617 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T209331 NZ_CP104030:c4792851-4792318 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT209331 WP_004150355.1 NZ_CP079145:c5364242-5364000 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT209331 NZ_CP104030:c4793128-4792862 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9