Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4784595..4785405 Replicon chromosome
Accession NZ_CP079145
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWY71_RS23240 Protein ID WP_004178461.1
Coordinates 4784595..4785128 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWY71_RS23245 Protein ID WP_002887278.1
Coordinates 4785139..4785405 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY71_RS23235 (4783426) 4783426..4784547 + 1122 WP_002887282.1 cupin domain-containing protein -
KWY71_RS23240 (4784595) 4784595..4785128 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWY71_RS23245 (4785139) 4785139..4785405 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWY71_RS23250 (4785508) 4785508..4786941 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KWY71_RS23255 (4786931) 4786931..4787614 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWY71_RS23260 (4787786) 4787786..4789171 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KWY71_RS23265 (4789189) 4789189..4789533 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209328 WP_004178461.1 NZ_CP079145:c4785128-4784595 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209328 NZ_CP104030:2023609-2023711 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209328 WP_002887278.1 NZ_CP079145:c4785405-4785139 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209328 NZ_CP104030:c2023718-2023573 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References