Detailed information of TA system TA209316

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 818404..819061
• Replicon: chromosome
• Accession: NZ_CP079145
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KWY71_RS04020
• Protein ID: WP_004181233.1
• Coordinates: 818651..819061 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KWY71_RS04015
• Protein ID: WP_002916312.1
• Coordinates: 818404..818670 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY71_RS03990 (813560)	813560..814993	-	1434	WP_004181234.1	6-phospho-beta-glucosidase	-
KWY71_RS03995 (815112)	815112..815840	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KWY71_RS04000 (815890)	815890..816201	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KWY71_RS04005 (816365)	816365..817024	+	660	WP_002916317.1	hemolysin III family protein	-
KWY71_RS04010 (817175)	817175..818158	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
KWY71_RS04015 (818404)	818404..818670	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KWY71_RS04020 (818651)	818651..819061	+	411	WP_004181233.1	protein YgfX	Toxin
KWY71_RS04025 (819068)	819068..819589	-	522	WP_004144730.1	flavodoxin FldB	-
KWY71_RS04030 (819690)	819690..820586	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KWY71_RS04035 (820609)	820609..821322	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KWY71_RS04040 (821328)	821328..823061	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T209316 WP_004181233.1 NZ_CP079145:818651-819061 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T209316 NZ_CP104022:c3297478-3297203 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT209316 WP_002916312.1 NZ_CP079145:818404-818670 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT209316 NZ_CP104022:c3297730-3297482 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41