Detailed information of TA system TA209315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 344707..345293
• Replicon: chromosome
• Accession: NZ_CP079145
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC06_CMC387MC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: KWY71_RS01575
• Protein ID: WP_002920800.1
• Coordinates: 344925..345293 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: KWY71_RS01570
• Protein ID: WP_004174006.1
• Coordinates: 344707..344928 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY71_RS01550 (340864)	340864..341790	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KWY71_RS01555 (341787)	341787..343064	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
KWY71_RS01560 (343061)	343061..343828	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KWY71_RS01565 (343830)	343830..344543	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KWY71_RS01570 (344707)	344707..344928	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KWY71_RS01575 (344925)	344925..345293	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KWY71_RS01580 (345566)	345566..346882	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KWY71_RS01585 (346989)	346989..347876	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KWY71_RS01590 (347873)	347873..348718	+	846	WP_004181473.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KWY71_RS01595 (348720)	348720..349790	+	1071	WP_043906884.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	304947..350527	45580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T209315 WP_002920800.1 NZ_CP079145:344925-345293 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T209315 NZ_CP104022:c3043307-3042888 [Erwinia amylovora]
GTGGTCCTGTGGCAATGTGAACTGCGGCCATCAAGGCTGGCACAGCGGTTTTCGCTGCTGCTGCATGGGGCAGTGATGCT
GGCGCTGCTATTGCCGACATGGCCCGCCAGCTCTGGCCTGGTGCGGATGCTGTTGCTGGTTTTGGTACTGTTGGAGTGCA
TACGCAGTCGGCGGCGCATTGGTCGTCGCCAGGGGGATATCGCTCTGTTGGAAGGGCATGAGCTACGCTGGCGTCAACGC
GAGTGGTGCATTATTTCCCGTCCGTGGCTGACCGGGCAGGCAATCCTGCTGTTACTACAGGATACTCATGGACAGCGCGA
GCGACTATGGCTGTTCGCCGACGGCATGGAAAAACGTAACTGGCGTCAGCTGCGCCTGCAATTGCTGAACAGTAAAGTAC
AGGGGAATGGCTGGTGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT209315 WP_004174006.1 NZ_CP079145:344707-344928 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT209315 NZ_CP104022:c3043554-3043288 [Erwinia amylovora]
ATGGATATTACAAATAAATCACGTATTCACTGGGCATGCCGTCGCGGCATGCGCGAGCTGGATATCTCGATCATGCCCTT
TTTCCAGTATGAATTTGACTCGCTGAACGACAGCGATAAACGGCTGTTCGTTCGCCTGCTGGAGAGCGATGACCCGGACC
TGTTCAACTGGCTGATGAACCACGGCGAGCCGGCAGACGTGGAGCTGAAAAGGATGGTGGCGCTAATCCAACAAAGAAAT
AAACAACGTGGTCCTGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7