Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ataRT/ElaA-DUF1778 |
| Location | 245089..245840 | Replicon | plasmid pCMC387P_P1 |
| Accession | NZ_CP079139 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC | ||
Toxin (Protein)
| Gene name | ataT | Uniprot ID | H6U1U8 |
| Locus tag | KWY70_RS27210 | Protein ID | WP_014386536.1 |
| Coordinates | 245089..245571 (-) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | ataR | Uniprot ID | A0A071LPN3 |
| Locus tag | KWY70_RS27215 | Protein ID | WP_004902250.1 |
| Coordinates | 245562..245840 (-) | Length | 93 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KWY70_RS27190 (KWY70_27190) | 241488..242135 | - | 648 | WP_014386537.1 | EcsC family protein | - |
| KWY70_RS27195 (KWY70_27195) | 242162..242917 | - | 756 | WP_004902235.1 | DUF2971 domain-containing protein | - |
| KWY70_RS27200 (KWY70_27200) | 243018..243410 | - | 393 | WP_032442757.1 | hypothetical protein | - |
| KWY70_RS27205 (KWY70_27205) | 243515..244054 | - | 540 | WP_004902239.1 | hypothetical protein | - |
| KWY70_RS27210 (KWY70_27210) | 245089..245571 | - | 483 | WP_014386536.1 | GNAT family N-acetyltransferase | Toxin |
| KWY70_RS27215 (KWY70_27215) | 245562..245840 | - | 279 | WP_004902250.1 | DUF1778 domain-containing protein | Antitoxin |
| KWY70_RS27220 (KWY70_27220) | 245959..246171 | - | 213 | WP_004902255.1 | hypothetical protein | - |
| KWY70_RS27225 (KWY70_27225) | 246279..246620 | - | 342 | WP_004902257.1 | hypothetical protein | - |
| KWY70_RS27230 (KWY70_27230) | 247450..247908 | - | 459 | WP_014386535.1 | hypothetical protein | - |
| KWY70_RS27235 (KWY70_27235) | 248561..249016 | - | 456 | WP_001166628.1 | Hg(II)-responsive transcriptional regulator | - |
| KWY70_RS27240 (KWY70_27240) | 249088..249453 | + | 366 | WP_004200999.1 | mercuric ion transporter MerT | - |
| KWY70_RS27245 (KWY70_27245) | 249469..249744 | + | 276 | WP_000732275.1 | mercury resistance system periplasmic binding protein MerP | - |
| KWY70_RS27250 (KWY70_27250) | 249772..250197 | + | 426 | WP_000522996.1 | organomercurial transporter MerC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..286022 | 286022 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17662.45 Da Isoelectric Point: 8.9130
>T209313 WP_014386536.1 NZ_CP079139:c245571-245089 [Klebsiella pneumoniae subsp. pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
Download Length: 483 bp
>T209313 NZ_CP104022:c1929866-1929495 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA
Antitoxin
Download Length: 93 a.a. Molecular weight: 10704.23 Da Isoelectric Point: 4.5271
>AT209313 WP_004902250.1 NZ_CP079139:c245840-245562 [Klebsiella pneumoniae subsp. pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
Download Length: 279 bp
>AT209313 NZ_CP104022:c1930084-1929863 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2A5MBI1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A071LPN3 |