Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | stbDE/ParE-YafN |
Location | 23055..23577 | Replicon | plasmid pCMC387P_P1 |
Accession | NZ_CP079139 | ||
Organism | Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC |
Toxin (Protein)
Gene name | stbE | Uniprot ID | A0A0C7KG00 |
Locus tag | KWY70_RS26050 | Protein ID | WP_038991638.1 |
Coordinates | 23293..23577 (+) | Length | 95 a.a. |
Antitoxin (Protein)
Gene name | stbD | Uniprot ID | A0A2J4R0U8 |
Locus tag | KWY70_RS26045 | Protein ID | WP_004181777.1 |
Coordinates | 23055..23303 (+) | Length | 83 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
KWY70_RS26025 (KWY70_26025) | 18264..19085 | - | 822 | WP_004181772.1 | hypothetical protein | - |
KWY70_RS26030 (KWY70_26030) | 19147..19500 | - | 354 | WP_004181774.1 | hypothetical protein | - |
KWY70_RS26035 (KWY70_26035) | 19645..20631 | - | 987 | WP_040120328.1 | hypothetical protein | - |
KWY70_RS26040 (KWY70_26040) | 20965..22764 | - | 1800 | WP_043907032.1 | ATP-dependent helicase | - |
KWY70_RS26045 (KWY70_26045) | 23055..23303 | + | 249 | WP_004181777.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
KWY70_RS26050 (KWY70_26050) | 23293..23577 | + | 285 | WP_038991638.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
KWY70_RS26055 (KWY70_26055) | 23594..23695 | - | 102 | Protein_27 | IS200/IS605 family transposase | - |
KWY70_RS26060 (KWY70_26060) | 23731..24957 | + | 1227 | WP_040120327.1 | RNA-guided endonuclease TnpB family protein | - |
KWY70_RS26065 (KWY70_26065) | 25229..25453 | - | 225 | Protein_29 | transposase | - |
KWY70_RS26070 (KWY70_26070) | 25532..25960 | - | 429 | WP_077256229.1 | IS200/IS605 family transposase | - |
KWY70_RS26075 (KWY70_26075) | 25996..27183 | + | 1188 | WP_043907031.1 | RNA-guided endonuclease TnpB family protein | - |
KWY70_RS29030 | 27228..27599 | - | 372 | WP_044816264.1 | hypothetical protein | - |
KWY70_RS29035 | 27596..27940 | - | 345 | WP_014386518.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 | - | 1..286022 | 286022 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10993.73 Da Isoelectric Point: 10.6516
>T209311 WP_038991638.1 NZ_CP079139:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN
Download Length: 285 bp
>T209311 NZ_CP104022:897781-898017 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 8925.17 Da Isoelectric Point: 3.8209
>AT209311 WP_004181777.1 NZ_CP079139:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL
Download Length: 249 bp
>AT209311 NZ_CP104022:897564-897791 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0C7KG00 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2J4R0U8 |