Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3137210..3137835 Replicon chromosome
Accession NZ_CP079138
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY70_RS15050 Protein ID WP_002882817.1
Coordinates 3137210..3137593 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY70_RS15055 Protein ID WP_004150355.1
Coordinates 3137593..3137835 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY70_RS15035 (3134576) 3134576..3135478 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWY70_RS15040 (3135475) 3135475..3136110 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY70_RS15045 (3136107) 3136107..3137036 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY70_RS15050 (3137210) 3137210..3137593 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY70_RS15055 (3137593) 3137593..3137835 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY70_RS15060 (3138040) 3138040..3138957 + 918 WP_004181612.1 alpha/beta hydrolase -
KWY70_RS15065 (3138971) 3138971..3139912 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY70_RS15070 (3139957) 3139957..3140394 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY70_RS15075 (3140391) 3140391..3141251 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWY70_RS15080 (3141245) 3141245..3141844 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209300 WP_002882817.1 NZ_CP079138:c3137593-3137210 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209300 NZ_CP104014:c4706812-4706501 [Enterobacter sp. CP102]
ATGCTGTTCATTGAAACGGAAATTTTCACTGAAGACGTCAAATTGTTACTGGATGACGATGAATACCGTAAACTTCAGGT
ATTCCTGGCTATGCAACCTGATTATGGCGATCTGATCCAGAATACAGGAGGCTTACGAAAGATTCGTTGGCTTGCTGGGG
GTAAGGGAAAGCGTGGTGGAGTCCGGGTCATCTATTTTCATCGTACGCGTGAGTTTGAAATTAGGTTACTGCTTATCTAC
CGCAAAGGAATCAAAGATGATCTGTCGGCAACTGAGAAAGCCGTACTGAAAAAGATTATTGAGAGGTGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209300 WP_004150355.1 NZ_CP079138:c3137835-3137593 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209300 NZ_CP104014:c4706500-4706210 [Enterobacter sp. CP102]
ATGGACAAGGCGCTGTTTGAGCGGTTAACCCAAAGCATGGCCCAAATGAATGAAATTGCCGAGGGCAGACGAGATCCGTC
GCGGACGTTTCAAATTGATGCGATGAAAATTAAAGAAATCCGTCAGGCGTCCGGATTGTCACAGAGTAAATTTGCAAATT
TAATCTCTGTCAGCGTCGATACCTTACGCAATTGGGAACAAGGCAGACGCTCACCCACAGGACCGGCTCAAGCACTGCTG
CGTGCAATTGCTAATGATCCTCAACATGTTATCCCTGCGCTCTCGCATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References