Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2558188..2558998 Replicon chromosome
Accession NZ_CP079138
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWY70_RS12375 Protein ID WP_004178461.1
Coordinates 2558188..2558721 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWY70_RS12380 Protein ID WP_002887278.1
Coordinates 2558732..2558998 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY70_RS12370 (2557019) 2557019..2558140 + 1122 WP_002887282.1 cupin domain-containing protein -
KWY70_RS12375 (2558188) 2558188..2558721 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWY70_RS12380 (2558732) 2558732..2558998 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWY70_RS12385 (2559101) 2559101..2560534 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KWY70_RS12390 (2560524) 2560524..2561207 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWY70_RS12395 (2561379) 2561379..2562764 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KWY70_RS12400 (2562782) 2562782..2563126 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T209297 WP_004178461.1 NZ_CP079138:c2558721-2558188 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209297 NZ_CP104014:2338776-2340101 [Enterobacter sp. CP102]
ATGCCAAAACTAGTGACGTGGATGAATAACGAACGGGTCGGCGAACTGACAAAGCTGACTAACGGAGCCCATACGTTTAA
ATATGCCAATGAATGGCTACTGAACCGGTATGCCAGGCCATTGTCGTTATCGCTTCCGCTGCAATTTGGTGAAATTACCT
CTGATGCGGTGTTCAATTTCTTTGACAACCTGCTCCCGGATAGCCCGGCGGTACGGGATCGTATTGTTAAGCGCTACCAC
GCCCGTTCCAGGCAACCCTTTGATTTACTGGCAGAGGTAGGGCGTGACAGCGTTGGCGCGGTGACGTTATTACCCGCAAA
TGAAAACAGTACGACACCGGAAATGACATGGGAGACACTGAGCGAAGAGAAGCTCGAGACGGTGCTGACGGCTTATCAGT
CAGACATTCCATTGGGGATGATTAATGAGGAAAATGATTTCCGCATTTCCGTTGCTGGCGCTCAGGAAAAAACGGCGCTC
CTTAAAGCCGGTGACGACTGGTGTATTCCGAAAGGGATCACGCCTACCACGCATATCATCAAGCTGCCGATTGGCGAGAT
TAAACAGCCTAATGCCACGCTGGATTTGAGCGACAGTGTGGATAATGAATACTTCTGTTTGCAGCTGGCCAGAGCGCTGA
GATTTGATGTGCCGAATGCAGAAATCATCAGAGCGGGCAGGGTGCGGGCACTTGCCGTCGAGCGTTTTGACCGTCGCTGG
AACGCAAGCCGCTCAGTTTTATTGCGATTACCGCAGGAAGATATGTGTCAGACTTTTGGGCTTCCTTCGTCCGTGAAGTA
TGAGTCTGATGGCGGCCCCGGTATTCCACACATCATGGCATTTTTACTGGGCTCCAGCGAAGCGCTTACCGATCGTTATA
ATTTCATGAAATTCCAGCTATTTCAGTGGATAACGGGGGCAACAGATGGACATGCCAAGAACTTCTCGATATTCATCCAG
CCTGGCGGAAGTTATCGACTGACCCCGTTCTACGACATCATTTCGGCGTTTCCTGTGCTGGGCGGCGCCGGGATCCACAT
CAGCGATCTGAAACTGGCCATGAGCCTGAATGCCTCAAAAGGCCGTAAAAACGATATTGACAAGATCTATCCGAGACATT
TTATGGCGACAGCCAGGGCCGTCAGGTTTCCTGAAAACCAGATGACCGGGATAATGCAGGAATTTATCGACCATGTGCCG
CAGGCGCTGGATACGGTGATGAGTTCTTTACCCACGGACTTTTCGCAAAAGGTCGCGACCTCGATATACAAGAATGTGCT
CAGACTGCATGGCCGGCTGAGTAAAAGTGCAGGGGGCTCAACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209297 WP_002887278.1 NZ_CP079138:c2558998-2558732 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209297 NZ_CP104014:2338507-2338776 [Enterobacter sp. CP102]
ATGAAACCACCAATGATTTATAGTCCGGTCCAGCTGGCGAATACCATGAGACTGGTGCGTCAGAAAAATAACTGGACGCA
AAGCGACCTGGCCAAAAGAATCGGCATTAAACAGGCCACCATCTCGAATTTTGAAAACAATCCGGATAACACCACGCTGA
CAACGTTCTTCAGGATCTTGCAATCCCTTGAAATGACCATGGCATTGTATGAAAAAGACGATACGGCGTCGGGGTCAGAC
AATAACAACCAACAGGGCCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References