209296

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1963612..1964231 Replicon chromosome
Accession NZ_CP079138
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC05_CMC387PC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWY70_RS09550 Protein ID WP_002892050.1
Coordinates 1964013..1964231 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWY70_RS09545 Protein ID WP_002892066.1
Coordinates 1963612..1963986 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY70_RS09535 (1958764) 1958764..1959957 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWY70_RS09540 (1959980) 1959980..1963126 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWY70_RS09545 (1963612) 1963612..1963986 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWY70_RS09550 (1964013) 1964013..1964231 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWY70_RS09555 (1964390) 1964390..1964956 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWY70_RS09560 (1964928) 1964928..1965068 - 141 WP_004147370.1 hypothetical protein -
KWY70_RS09565 (1965089) 1965089..1965559 + 471 WP_002892026.1 YlaC family protein -
KWY70_RS09570 (1965534) 1965534..1966985 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KWY70_RS09575 (1967086) 1967086..1967784 + 699 WP_004178762.1 GNAT family protein -
KWY70_RS09580 (1967781) 1967781..1967921 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWY70_RS09585 (1967921) 1967921..1968184 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209296 WP_002892050.1 NZ_CP079138:1964013-1964231 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209296 NZ_CP104014:c1164863-1164501 [Enterobacter sp. CP102]
ATGTGGGACATCGAGACAACGGACAGATTTGATAAATGGTTTTTTAACCAGACACTCGCATTAAAGGAGGACGTACTTGC
TGCAATGCATATTCTCGCTGAGTTTGGCCCTCAGCTTGGGCGTCCATATGTCGATACCGTAAAAGCTTCAGCCTATCCGA
ACATGAAAGAGCTGCGGATCCAGCATTCAGGCGATCCTATTAGAGCCTTTTTTGCTTTTGATCCACGTCGCACCGCCATT
GTGTTGTGTGCCGGAGACAAGAGCGGATGTGTTGAGAAGCGATTCTATAATGAGATGATTAAGCTAGCTGATGCTGAATT
CAGCAAACATCTGGCAAATAAGGAGGCCATATGGCATCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209296 WP_002892066.1 NZ_CP079138:1963612-1963986 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209296 NZ_CP104014:c1164513-1164214 [Enterobacter sp. CP102]
ATGGCATCGCTAAAAGAACTTATGGCGCAACAGAGTGCAGAAAGTCAGGAGCGTATCGCGACGAAAGTTGAAAAGATGCG
CCGAATTGTTGCCCTTAATCAACTGCGGGAAGAACTGAGCGTTTCACAAACGGAGCTCGCTGCGGCAATGGGGGTAAAGC
AACCCACAGTGGCAAAAATCGAACAACCGGGCAACGATCCCCGACTCTCAACACTCAAACGCTATGTCAGTGCGCTTGGG
GGAGAACTCAGTATTGATGTGACGCTACCGAATGGAAAACGGATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References