Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4789274..4789893 Replicon chromosome
Accession NZ_CP079131
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWY69_RS23290 Protein ID WP_002892050.1
Coordinates 4789675..4789893 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWY69_RS23285 Protein ID WP_002892066.1
Coordinates 4789274..4789648 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY69_RS23275 (4784426) 4784426..4785619 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWY69_RS23280 (4785642) 4785642..4788788 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWY69_RS23285 (4789274) 4789274..4789648 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWY69_RS23290 (4789675) 4789675..4789893 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWY69_RS23295 (4790052) 4790052..4790618 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWY69_RS23300 (4790590) 4790590..4790730 - 141 WP_004147370.1 hypothetical protein -
KWY69_RS23305 (4790751) 4790751..4791221 + 471 WP_002892026.1 YlaC family protein -
KWY69_RS23310 (4791196) 4791196..4792647 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KWY69_RS23315 (4792748) 4792748..4793446 + 699 WP_004178762.1 GNAT family protein -
KWY69_RS23320 (4793443) 4793443..4793583 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWY69_RS23325 (4793583) 4793583..4793846 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209289 WP_002892050.1 NZ_CP079131:4789675-4789893 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209289 NZ_CP104011:c1978205-1977921 [Pseudomonas sp. GCEP-101]
ATGACCTATGAGCTGGAGTTTCTCCCCGCCGCGCTGAAGGAGTGGCGCAAGCTGGGCCATACGGTGCGGGAGCAATTCAA
ACGCAAACTCGCCGAGCGCCTGGAAGAACCTCGCGTACCGGCCGACGCCCTGCACGGGCTGGCGGACCACTACAAGATCA
AGCTGCGCTCGGCAGGCTACCGTCTGGTCTATCGCGTCGAGGATGAGCGCGTGGTGATTGCCGTCGTTGCGGTTGGCAAA
CGCGAACGTGGCGAGGTGTATAGCGCGGCGCAGGACCGGCGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209289 WP_002892066.1 NZ_CP079131:4789274-4789648 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209289 NZ_CP104011:c1978440-1978195 [Pseudomonas sp. GCEP-101]
ATGCAGAATCTACTTGCCAGTACGGCCGTCAGCGTCTCGGAACTGAAGAAGAACCCCTCCGCCGTGCTGGCGGAATCCGG
TGGCGAGCCGGTGGCGGTGCTCAACCACAACAAGGTGATGGGCTACATGGTGCCGGCGGAGCTGTTCGAGTCGATCCTGG
AGCGCCTGGAAGACCTGGAGCTGGCGGAAATCGCCCGCTCGCGACTGGGAGAAAAAGGCATCCCGGTATCGCTCGATGAC
CTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References