Detailed information of TA system TA209287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3453121..3454709
• Replicon: chromosome
• Accession: NZ_CP079131
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KWY69_RS16970
• Protein ID: WP_004179815.1
• Coordinates: 3453121..3454443 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KWY69_RS16975
• Protein ID: WP_004176198.1
• Coordinates: 3454443..3454709 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY69_RS16955 (3448177)	3448177..3450678	+	2502	WP_004179823.1	fimbria/pilus outer membrane usher protein	-
KWY69_RS16960 (3450666)	3450666..3451670	+	1005	WP_004179822.1	fimbrial protein	-
KWY69_RS16965 (3451812)	3451812..3453104	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KWY69_RS16970 (3453121)	3453121..3454443	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
KWY69_RS16975 (3454443)	3454443..3454709	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KWY69_RS16980 (3454975)	3454975..3455301	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
KWY69_RS16985 (3455298)	3455298..3455798	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KWY69_RS16990 (3455776)	3455776..3457155	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
KWY69_RS16995 (3457501)	3457501..3458655	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KWY69_RS17000 (3458636)	3458636..3459559	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T209287 WP_004179815.1 NZ_CP079131:c3454443-3453121 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T209287 NZ_CP104011:940767-941174 [Pseudomonas sp. GCEP-101]
GTGAAGTACCTGCTCGACACCAATATCCTCATCTACCTGCTGAAGAACCGGCCGGAATCCGTCGCGCAACGGGTTAATGC
CCTGCCCGCCGACGCCTCGCTGAACATGTCGTTCTTCACCTATGCCGAGCTGCTAAAAGGTGCCGAACGCAGCTCGCGCA
AGGCCGAAGTCCTGCGCCAGCTGGACCGCCTGACGCGTCAGGTGCCGGTAATCTACGACGGCACCGCCCGCCTGTGCGAA
CACTACGCCGTGCACTTCACCCGGCTGAAGCAGGCCGGCACGCCCATCGGGGCCAATGATCTGTGGATCGCCTGCCACGC
TCTCGCGCTCGACGCCACGCTGGTCACGCACAACACCCGCGAGTTCGAGCGTATCGACGGCCTGATGCTGGAAGACTGGG
CAAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT209287 WP_004176198.1 NZ_CP079131:c3454709-3454443 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT209287 NZ_CP104011:940510-940770 [Pseudomonas sp. GCEP-101]
ATGCCGACTTCCACCTTGACTACCCGCGTGTTCATGAACGGCAACAGCCAAGCCGTGCGGATTCCCCAGGAGTTCCGCCT
GGATGTTGGCAGCGTGGAGATCACCCGCAAGGACAACGGCGACCTGGTGATCCATCCCATCCCGCAGGACCGTGGCGCCG
CGCTGATGGAAGCGCTGCAGGGCTTCGACGAAGACTTCCTCGAGGCCCTGGACGACGAACAGCGCGACCAGCCACCGGCC
CAGGACCGGGACGAGCTGTGA