Detailed information of TA system TA209278

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1542817..1543474
• Replicon: chromosome
• Accession: NZ_CP079131
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KWY69_RS07455
• Protein ID: WP_004181233.1
• Coordinates: 1543064..1543474 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KWY69_RS07450
• Protein ID: WP_002916312.1
• Coordinates: 1542817..1543083 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWY69_RS07425 (1537973)	1537973..1539406	-	1434	WP_004181234.1	6-phospho-beta-glucosidase	-
KWY69_RS07430 (1539525)	1539525..1540253	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KWY69_RS07435 (1540303)	1540303..1540614	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KWY69_RS07440 (1540778)	1540778..1541437	+	660	WP_002916317.1	hemolysin III family protein	-
KWY69_RS07445 (1541588)	1541588..1542571	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
KWY69_RS07450 (1542817)	1542817..1543083	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KWY69_RS07455 (1543064)	1543064..1543474	+	411	WP_004181233.1	protein YgfX	Toxin
KWY69_RS07460 (1543481)	1543481..1544002	-	522	WP_004144730.1	flavodoxin FldB	-
KWY69_RS07465 (1544103)	1544103..1544999	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KWY69_RS07470 (1545022)	1545022..1545735	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KWY69_RS07475 (1545741)	1545741..1547474	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T209278 WP_004181233.1 NZ_CP079131:1543064-1543474 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T209278 NZ_CP104006:1364849-1365190 [Yersinia alsatica]
ATGAAACCTCAATCTGCAACAACTTCGCGGGTGTTAAAACTCTGCCTGTCACCCGTGGCTATCTGGCAAATGCTTCTGAC
ACGCCTGCTGGAACAACACTATGGCCTGATGCTAAAGGACACACCTTTCAGTGATGAAATCGTTATTCAGAAACATATCG
ATGCAGGTATCACGCTGGCCGATGCCGTTAATTTTCTAGTGGAAAAAGACGAGCTGGTTCGAATAGATCGCAAAGGATTT
AGCTGGCAGGAACAATCCCCTTATCTTCGGGCTGTTGATATTCTGCGAGCGCGACAGGCAACTGGCTTGTTGCAGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT209278 WP_002916312.1 NZ_CP079131:1542817-1543083 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT209278 NZ_CP104006:1364511-1364828 [Yersinia alsatica]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATCACGCCACGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGGGTGCCGAAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATTCATGTTGACCACCGGTGAAATGAATCCTCGTCACGCCCACTGCGTCATCCTGTACCAC
AACGGTTTTACCTGCGAAGCCCATACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCTACTCAGCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41