Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 600753..601378 Replicon chromosome
Accession NZ_CP079131
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC04_AI1235MC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWY69_RS02775 Protein ID WP_002882817.1
Coordinates 600753..601136 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWY69_RS02780 Protein ID WP_004150355.1
Coordinates 601136..601378 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWY69_RS02760 (598119) 598119..599021 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWY69_RS02765 (599018) 599018..599653 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWY69_RS02770 (599650) 599650..600579 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KWY69_RS02775 (600753) 600753..601136 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWY69_RS02780 (601136) 601136..601378 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWY69_RS02785 (601583) 601583..602500 + 918 WP_004181612.1 alpha/beta hydrolase -
KWY69_RS02790 (602514) 602514..603455 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWY69_RS02795 (603500) 603500..603937 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KWY69_RS02800 (603934) 603934..604794 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWY69_RS02805 (604788) 604788..605387 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209276 WP_002882817.1 NZ_CP079131:c601136-600753 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209276 NZ_CP104006:c57280-56960 [Yersinia alsatica]
ATGGATAAGCATAAAAAGAGAAGTATCGGCAGTTTTCGCGATACGTGGCTTGAGGGTTTCTTCGAGCACGGCGGCAGGCA
CCGAAAAATACCCGCAAGTATAGAAAATGTGCTTGCCAGAAAGCTGGATATTATAAACGCCGCAATAAGTTATAAAGATT
TACGCTCACCCCCCGCCAATCGTTATGAGGAGCTGAATCCGCCATTAGAAGGCTACTCTTCTATTCGTATTAATGATCAG
TACCGACTTATTTTTGTGTGGGCGAATGGCAAGGCCAACGATTTATACCTTGATGCGCATGGTTACAAGAAGCACAGATA
G

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209276 WP_004150355.1 NZ_CP079131:c601378-601136 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209276 NZ_CP104006:c56910-56572 [Yersinia alsatica]
ATGGTCCAAGCACAGCGCAAGCCAACCTCTGTTGGTGACGTCTTGTTGTATGAATATATTGAGCCGACAGGCATAAAAAT
TAATGATCTGGCGGAAATGCTTCAGGTGCATAGGAATACCATCAGCGCCTTGGTTAACAATAACCGCAAACTGACCACAG
ATATGGCATTTAGATTAGCTAAAGCATTTGATACTTCTGAAGAGTTTTGGATAAATCTTCAGGCCAATGTTGATATTTGG
GAAGTACAAAATGACAACCGAATTCAAGAAGAGCTGAGTCGGGTAATCACATTAGATGATTTCCTTAAACGGAAAGAAAA
ATCTAAAAAATTAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References