Detailed information of TA system TA209272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 60..534
• Replicon: plasmid pAI1235P_P9
• Accession: NZ_CP079129
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC03_AI1235PC

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: KWV87_RS28705
• Protein ID: WP_000262326.1
• Coordinates: 60..326 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: KWV87_RS28710
• Protein ID: WP_000879771.1
• Coordinates: 310..534 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWV87_RS28705 (KWV87_28705)	60..326	-	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KWV87_RS28710 (KWV87_28710)	310..534	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
KWV87_RS28715 (KWV87_28715)	1080..1268	+	189	WP_000165998.1	Rop family plasmid primer RNA-binding protein	-
KWV87_RS28720 (KWV87_28720)	1316..1543	-	228	WP_224019247.1	hypothetical protein	-
KWV87_RS28725 (KWV87_28725)	2688..3119	+	432	WP_053274236.1	DUF6530 family protein	-
KWV87_RS28730 (KWV87_28730)	3211..3690	+	480	WP_227525340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..3692	3692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T209272 WP_000262326.1 NZ_CP079129:c326-60 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T209272 NZ_CP104001:c1069814-1069596 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACTAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT209272 WP_000879771.1 NZ_CP079129:c534-310 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT209272 NZ_CP104001:c1070216-1069842 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5