Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58669..59405 Replicon plasmid pAI1235P_P1
Accession NZ_CP079121
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC03_AI1235PC

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KWV87_RS26000 Protein ID WP_003026803.1
Coordinates 58923..59405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KWV87_RS25995 Protein ID WP_003026799.1
Coordinates 58669..58935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWV87_RS25950 (KWV87_25950) 54731..55093 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KWV87_RS25955 (KWV87_25955) 55143..55493 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KWV87_RS25960 (KWV87_25960) 55851..56120 + 270 WP_004152102.1 hypothetical protein -
KWV87_RS25965 (KWV87_25965) 56108..56683 + 576 WP_004152103.1 hypothetical protein -
KWV87_RS25970 (KWV87_25970) 56714..57208 + 495 WP_004152104.1 DNA-binding protein -
KWV87_RS25975 (KWV87_25975) 57252..57620 + 369 WP_004152105.1 hypothetical protein -
KWV87_RS25980 (KWV87_25980) 57654..57857 + 204 WP_004152106.1 HHA domain-containing protein -
KWV87_RS25985 (KWV87_25985) 57906..58163 + 258 WP_004152107.1 hypothetical protein -
KWV87_RS25990 (KWV87_25990) 58239..58493 + 255 WP_004152108.1 hypothetical protein -
KWV87_RS25995 (KWV87_25995) 58669..58935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KWV87_RS26000 (KWV87_26000) 58923..59405 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KWV87_RS26005 (KWV87_26005) 59606..61009 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KWV87_RS26010 (KWV87_26010) 61038..61670 - 633 WP_001567369.1 hypothetical protein -
KWV87_RS26015 (KWV87_26015) 61900..63246 + 1347 WP_020314316.1 ISNCY family transposase -
KWV87_RS29070 63407..63538 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..198536 198536


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T209268 WP_003026803.1 NZ_CP079121:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T209268 NZ_CP104000:c16505-16221 [Ketogulonicigenium vulgare]
ATGAGCTATAAACTTGAGTTCCTGCCTTCGGCTCTCAAAGAGTGGAGGAAGCTTGATTCAGTGATCGCCGATCAGTTCAG
ACGTAAGCTATTGAAATTAAGAGATAACCCACGGGTTTCCTCATTAGCTTTGCGTAACCTAAAGGACTGTTACAAAGTTA
AGTTGGCAAGCGCAGGTTTCAGACTTGTCTACCAAGTGGTGGATGACCGGATGATCGTCCAAGTCGTCGCTGTAGGAAAG
CGCTCTAACGGGGACGTGTACGACACTGCCTCCAAACGAGTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT209268 WP_003026799.1 NZ_CP079121:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209268 NZ_CP104000:c16740-16495 [Ketogulonicigenium vulgare]
TTGCAGCGGGTTAGTGCTGGCTTGGCGATGAGCATATCCGATTTGAAGAAGAACCCCTCAGCGGCCTTCGACGTCTCCGG
TGGACAGCCAGTAGCGATCCTTAACCACAACAAGGTCGTGGGCTACATGGTAACCCCCGATCGATATGAGGCCCTACTCG
AGGCTCTGGATGATGCTCGTCTCGCCGAGACCATTCGTGAGCGCTCTGGGGAGGTGCCTGTCGCACTGGACTGGAATGAG
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References