Detailed information of TA system TA209249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 161349..161874
• Replicon: plasmid pS465_P1
• Accession: NZ_CP079116
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: KWK18_RS27840
• Protein ID: WP_001159868.1
• Coordinates: 161349..161654 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: KWK18_RS27845
• Protein ID: WP_000813634.1
• Coordinates: 161656..161874 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWK18_RS27825 (KWK18_27825)	157259..158425	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
KWK18_RS27830 (KWK18_27830)	159013..159768	-	756	WP_000852146.1	replication initiation protein RepE	-
KWK18_RS27835 (KWK18_27835)	160542..161348	-	807	WP_000016982.1	site-specific integrase	-
KWK18_RS27840 (KWK18_27840)	161349..161654	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
KWK18_RS27845 (KWK18_27845)	161656..161874	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KWK18_RS27850 (KWK18_27850)	162439..162669	+	231	WP_016338373.1	type II toxin-antitoxin system VapB family antitoxin	-
KWK18_RS27855 (KWK18_27855)	162666..163082	+	417	WP_048983057.1	type II toxin-antitoxin system VapC family toxin	-
KWK18_RS28435	163123..163446	-	324	WP_085842301.1	hypothetical protein	-
KWK18_RS28440	163464..164000	-	537	WP_223825639.1	hypothetical protein	-
KWK18_RS27865 (KWK18_27865)	164659..165936	+	1278	WP_016338369.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-4 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / tet(D) / blaTEM-1B	-	1..210997	210997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T209249 WP_001159868.1 NZ_CP079116:c161654-161349 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T209249 NZ_CP103974:3673429-3673647 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT209249 WP_000813634.1 NZ_CP079116:c161874-161656 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT209249 NZ_CP103974:3673029-3673403 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2