Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5415566..5416191 Replicon chromosome
Accession NZ_CP079115
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWK18_RS26535 Protein ID WP_002882817.1
Coordinates 5415566..5415949 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWK18_RS26540 Protein ID WP_004150355.1
Coordinates 5415949..5416191 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWK18_RS26520 (5412932) 5412932..5413834 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWK18_RS26525 (5413831) 5413831..5414466 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWK18_RS26530 (5414463) 5414463..5415392 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KWK18_RS26535 (5415566) 5415566..5415949 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWK18_RS26540 (5415949) 5415949..5416191 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWK18_RS26545 (5416396) 5416396..5417313 + 918 WP_004181612.1 alpha/beta hydrolase -
KWK18_RS26550 (5417327) 5417327..5418268 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWK18_RS26555 (5418313) 5418313..5418750 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KWK18_RS26560 (5418747) 5418747..5419607 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWK18_RS26565 (5419601) 5419601..5420200 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209247 WP_002882817.1 NZ_CP079115:c5415949-5415566 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209247 NZ_CP103974:2887831-2887938 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209247 WP_004150355.1 NZ_CP079115:c5416191-5415949 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209247 NZ_CP103974:c2887784-2887718 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References