Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4198415..4199034 Replicon chromosome
Accession NZ_CP079115
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC01_S465PC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWK18_RS20760 Protein ID WP_002892050.1
Coordinates 4198816..4199034 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWK18_RS20755 Protein ID WP_002892066.1
Coordinates 4198415..4198789 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWK18_RS20745 (4193567) 4193567..4194760 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWK18_RS20750 (4194783) 4194783..4197929 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWK18_RS20755 (4198415) 4198415..4198789 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWK18_RS20760 (4198816) 4198816..4199034 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWK18_RS20765 (4199197) 4199197..4199763 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWK18_RS20770 (4199735) 4199735..4199875 - 141 WP_004147370.1 hypothetical protein -
KWK18_RS20775 (4199896) 4199896..4200366 + 471 WP_002892026.1 YlaC family protein -
KWK18_RS20780 (4200341) 4200341..4201792 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
KWK18_RS20785 (4201893) 4201893..4202591 + 699 WP_004177238.1 GNAT family protein -
KWK18_RS20790 (4202588) 4202588..4202728 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWK18_RS20795 (4202728) 4202728..4202991 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209244 WP_002892050.1 NZ_CP079115:4198816-4199034 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209244 NZ_CP103974:2175464-2175567 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209244 WP_002892066.1 NZ_CP079115:4198415-4198789 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209244 NZ_CP103974:c2175605-2175334 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References