Detailed information of TA system TA209227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1297004..1297921
• Replicon: chromosome
• Accession: NZ_CP079099
• Organism: Bacillus velezensis strain LBY-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KVY05_RS06875
• Protein ID: WP_032866111.1
• Coordinates: 1297175..1297921 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KVY05_RS06870
• Protein ID: WP_003154807.1
• Coordinates: 1297004..1297174 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVY05_RS06835 (1292216)	1292216..1293805	+	1590	WP_205164551.1	hypothetical protein	-
KVY05_RS06840 (1293818)	1293818..1294243	+	426	WP_050498185.1	hypothetical protein	-
KVY05_RS06845 (1294248)	1294248..1294445	+	198	WP_007610833.1	XkdX family protein	-
KVY05_RS06850 (1294502)	1294502..1295263	+	762	WP_020955694.1	hypothetical protein	-
KVY05_RS06855 (1295315)	1295315..1295578	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KVY05_RS06860 (1295592)	1295592..1295855	+	264	WP_003154813.1	phage holin	-
KVY05_RS06865 (1295869)	1295869..1296747	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
KVY05_RS21760 (1296782)	1296782..1296907	-	126	WP_023356844.1	hypothetical protein	-
KVY05_RS06870 (1297004)	1297004..1297174	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KVY05_RS06875 (1297175)	1297175..1297921	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KVY05_RS06880 (1298025)	1298025..1299023	-	999	WP_020955696.1	inorganic phosphate transporter	-
KVY05_RS06885 (1299036)	1299036..1299653	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KVY05_RS06890 (1299939)	1299939..1301255	-	1317	WP_007407254.1	amino acid permease	-
KVY05_RS06895 (1301578)	1301578..1302528	+	951	WP_218791607.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1262000..1306178	44178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T209227 WP_032866111.1 NZ_CP079099:c1297921-1297175 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T209227 NZ_CP103972:3233280-3233453 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT209227 WP_003154807.1 NZ_CP079099:c1297174-1297004 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT209227 NZ_CP103972:3232956-3233252 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14