Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485007..485644 Replicon chromosome
Accession NZ_CP079099
Organism Bacillus velezensis strain LBY-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KVY05_RS02470 Protein ID WP_003156187.1
Coordinates 485294..485644 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag KVY05_RS02465 Protein ID WP_003156188.1
Coordinates 485007..485288 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVY05_RS02445 (481372) 481372..481971 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
KVY05_RS02450 (482064) 482064..482429 + 366 WP_007410229.1 holo-ACP synthase -
KVY05_RS02455 (482594) 482594..483601 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
KVY05_RS02460 (483718) 483718..484887 + 1170 WP_025284272.1 alanine racemase -
KVY05_RS02465 (485007) 485007..485288 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KVY05_RS02470 (485294) 485294..485644 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KVY05_RS02475 (485762) 485762..486583 + 822 WP_003156182.1 STAS domain-containing protein -
KVY05_RS02480 (486588) 486588..486953 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
KVY05_RS02485 (486956) 486956..487357 + 402 WP_003156178.1 anti-sigma regulatory factor -
KVY05_RS02490 (487369) 487369..488376 + 1008 WP_052827076.1 PP2C family protein-serine/threonine phosphatase -
KVY05_RS02495 (488440) 488440..488769 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
KVY05_RS02500 (488766) 488766..489248 + 483 WP_003156173.1 anti-sigma B factor RsbW -
KVY05_RS02505 (489214) 489214..490002 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
KVY05_RS02510 (490002) 490002..490604 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T209226 WP_003156187.1 NZ_CP079099:485294-485644 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T209226 NZ_CP103972:c2522253-2522146 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT209226 WP_003156188.1 NZ_CP079099:485007-485288 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT209226 NZ_CP103972:2522301-2522367 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References